Encontrados 5 documentos, a visualizar página 1 de 1
Proposal for a method to estimate nutrient shock effects in bacteria
Azevedo, N. F.; Bragança, S. M.; Simões, Lúcia C.; Cerqueira, L.; Almeida, Carina; Keevil, C. W.; Vieira, M. J.Plating methods are still the golden standard in microbiology; however, some studies have shown that these techniques can underestimate the microbial concentrations and diversity. A nutrient shock is one of the mechanisms proposed to explain this phenomenon. In this study, a tentative method to assess nutrient shock effects was tested. Findings To estimate the extent of nutrient shock effects, two strains is...
Use of fluorescent in situ hybridisation for the visualisation of Helicobacter ...
Bragança, S. M.; Azevedo, N. F.; Simões, Lúcia C.; Keevil, C. W.; Vieira, M. J.A fluorescently labelled peptide nucleic acid (PNA) probe has been applied for the in situ detection of Helicobacter pylori in drinking water biofilms. The method was originally applied to real pipe samples removed from a drinking water distribution system (DWDS) but the curvature and the heavy fouling of the pipes prevented an accurate detection of the bacterium by epifluorescence microscopy. Therefore, two se...
Detection and estimation of nutrient shock effects in bacteria
Azevedo, N. F.; Bragança, S. M.; Simões, Lúcia C.; Keevil, C. W.; Vieira, M. J.In this work we have synthesized TiO2 nanoparticles, using either a sol–gel base catalysed process in the interior of CTAB reversed micelles (TiO2 CTAB sol), or the neutralization of a TiO2/H2SO4 solution in the interior of AOT reversed micelles. From the absorption and emission data of the TiO2 nanoparticles it is possible to conclude that in the sol–gel route there remains alkoxide groups in the structure, or...
Use of fluorescent in situ hybridization for the visualization of Helicobacter ...
Bragança, S. M.; Azevedo, N. F.; Simões, Lúcia C.; Keevil, C. W.; Vieira, M. J.A fluorescently labelled peptide nucleic acid (PNA) probe has been applied for the in situ detection of Helicobacter pylori in drinking water biofilms. The method was originally applied to real pipe samples removed from a drinking water distribution system (DWDS) but the curvature and the heavy fouling of the pipes prevented an accurate detection of the bacterium by epifluorescence microscopy. Therefore, two se...
Detection of H. pylori in biofilms formed in a real drinking water distribution...
Bragança, S. M.; Azevedo, N. F.; Simões, Lúcia C.; Vieira, M. J.; Keevil, C. W.Helicobacter pylori has previously been detected in real drinking water distribution systems (DWDS) using PCR based- techniques, but this approach will not discriminate between live and dead cells and their spatial relationship within the biofilm. On the other hand, in situ detection using fluorescence in situ hybridization (FISH) has been used successfully for spatial resolution but only in lab-grown experimen...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||