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Expression of an exogenous 1-aminocyclopropane-1-carboxylate deaminase gene in ...
Brígido, Clarisse; Nascimento, Francisco X.; Duan, Jin; Glick, Bernard R.; Oliveira, SolangeOur goal was to study the symbiotic performance of two Mesorhizobium ciceri strains, transformed with an exogenous 1-aminocyclopropane-1-carboxylate deaminase gene (acdS), in chickpea plants under salinity stress. The EE-7 (salt-sensitive) and G-55 (salt-tolerant) M. ciceri strains were transformed with an acdS gene present on plasmid pRKACC. Salinity significantly reduced the overall growth of plants inoculate...
Most Acid-Tolerant Chickpea Mesorhizobia Show Induction of Major Chaperone Gene...
Brígido, Clarisse; Oliveira, SolangeOur goals were to evaluate the tolerance of mesorhizobia to acid and alkaline conditions as well as to investigate whether acid tolerance is related to the species or the origin site of the isolates. In addition, to investigate the molecular basis of acid tolerance, the expression of chaperone genes groEL and dnaKJ was analyzed using acid-tolerant and sensitive mesorhizobia. Tolerance to pH 5 and 9 was evaluate...
Enhanced chickpea growth-promotion ability of a Mesorhizobium strain expressing...
Nascimento, Francisco; Brígido, Clarisse; Alho, Luís; Glick, Bernard; Oliveira, SolangeAims The main goal of the study reported herein was to assess the nodulation performance of a Mesorhizobium strain transformed with an exogenous ACC deaminase gene (acdS), and its subsequent ability to increase chickpea plant growth under normal and waterlogged conditions. Methods The Mesorhizobium ciceri strain LMS-1 was transformed with the acdS gene of Pseudomonas putida UW4 by triparental conjugation using...
Mesorhizobium ciceri LMS-1 expressing an exogenous 1-aminocyclopropane-1-carbox...
Nascimento, Francisco; Brígido, Clarisse; Glick, Bernard; Oliveira, Solange; Alho, LuísAims: Our goal was to understand the symbiotic behaviour of a Mesorhizobium strain expressing an exogenous 1-aminocyclopropane-1-carboxylate (ACC) deaminase, which was used as an inoculant of chickpea (Cicer arietinum) plants growing in soil. Methods and Results: Mesorhizobium ciceri LMS-1 (pRKACC) was tested for its plant growth promotion abilities on two chickpea cultivars (ELMO and CHK3226) growing in nonste...
Transcriptional analysis of major chaperone genes in salt-tolerant and salt-sen...
Brígido, Clarisse; Alexandre, Ana; Oliveira, SolangeSalinity is an important abiotic stress that limits rhizobia-legume symbiosis, affecting plant growth, thus reducing crop productivity. Our aims were to evaluate the tolerance to salinity of native chickpea rhizobia as well as to investigate the expression of chaperone genes groEL, dnaKJ and clpB in both tolerant and sensitive isolates. One hundred and six native chickpea mesorhizobia were screened for salinity...
A ClpB Chaperone Knockout Mutant of Mesorhizobium ciceri Shows a Delay in the R...
Brígido, Clarisse; Robledo, Marta; Menéndez, Esther; Mateos, Pedro F.; Oliveira, SolangeSeveral molecular chaperones are known to be involved in bacteria stress response. To investigate the role of chaperone ClpB in rhizobia stress tolerance as well as in the rhizobiaplant symbiosis process, the clpB gene from a chickpea microsymbiont, strain Mesorhizobium ciceri LMS-1, was identified and a knockout mutant was obtained. The ClpB knockout mutant was tested to several abiotic stresses, showing that ...
ACC deaminase genes are conserved among Mesorhizobium species able to nodulate ...
Nascimento, Francisco X.; Brígido, Clarisse; Glick, Bernard R.; Oliveira, SolangeRhizobia strains expressing the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase have been reported to display an augmented symbiotic performance as a consequence of lowering the plant ethylene levels that inhibit the nodulation process. Genes encoding ACC deaminase (acdS) have been studied in Rhizobium spp.; however, not much is known about the presence of acdS genes in Mesorhizobium spp. The aim of th...
Survey of Chickpea Rhizobia Diversity in Portugal Reveals the Predominance of S...
Alexandre, Ana; Brígido, Clarisse; Laranjo, Marta; Rodrigues, Sérgio; Oliveira, SolangeSeveral Mesorhizobium species are able to induce effective nodules in chickpea, one of the most important legumes worldwide. Our aims were to examine the biogeography of chickpea rhizobia, to search for a predominant species, and to identify the most efficient microsymbiont, considering Portugal as a case study. One hundred and ten isolates were obtained from continental Portugal and Madeira Island. The 16S rib...
Moderately acidophilic Mesorhizobia isolated from chickpea (Cicer arietinum L.)
Brígido, Clarisse; Alexandre, Ana; Laranjo, Marta; Oliveira, SolangeAims: Our aim was to evaluate the effect of acid and alkaline pH on chickpea rhizobia, and on chickpea–rhizobia symbiosis. Methods and Results: Forty-seven rhizobia isolates obtained from 12 Portuguese soils were grown at pH 5, 7 and 9. Among these, 26 grew more at pH 5 than at 7, suggesting the existence of acidophiles. All isolates were identified as mesorhizobia by 16S rDNA partial sequence analysis. Molecu...
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