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Low metabolic activity of biofilm formed by Enterococcus faecalis isolated from...

It is widely known that Enterococcus faecalis virulence is related to its biofilm formation. Although Enterococci are common commensal organisms of the gastrointestinal tract, the difference between commensal and pathogen strains remain unclear. In this study, we compare the biochemical profile of the biofilms formed by two groups of medical and two groups of commensal strains. The medical strains were isolated...

Biological evaluation of alginate-based hydrogels, with antimicrobial features ...

In this work three different hydrogels were developed to associate, as vehicles, with the synthetic bone substitute GR-HA. One based on an alginate matrix (Alg); a second on a mixture of alginate and chitosan (Alg/Ch); and a third on alginate and hyaluronate (Alg/HA), using Ca2+ ions as cross-linking agents. The hydrogels, as well as the respective injectable bone substitutes (IBSs), were fully characterized fr...

Differential adherence and expression of virulence traits by Candida albicans a...

AIMS: To evaluate specific virulence factors of Candida albicans and Candida parapsilosis clinical oral isolates in mono- and dual-species culture in the presence of artificial saliva. METHODS AND RESULTS: Two of the strains used in this study were isolated from co-infection (C. albicans AM and C. parapsilosis AM2), and the other two were isolated from single infection (C. albicans AC and C. parapsilosis AD)...

Candida bracarensis virulence factors

Infective endocarditis in intravenous drug abusers : an update

Infective endocarditis despite advances in diagnosis remains a common cause of hospitalization, with high morbidity and mortality rates. Through literature review it is possible to conclude that polymicrobial endocarditis occurs mainly in intravenous drug abusers with predominance in the right side of the heart, often with tricuspid valve involvement. This fact can be associated with the type of drug used by th...

Adhesion and enzymatic activity of co-cultures of C. albicans and C. parapsilos...

An in vitro evaluation of Candida tropicalis infectivity using human cell monol...

The aim of the present study was to investigate the interaction ofCandida tropicalis with three different human cell lines: TCC-SUP (epithelial cells from urinary bladder); HeLa (epithelial cells from cervical carcinoma); Caco-2 (epithelial cells from colorectal adenocarcinoma). In particular to assess the degree of cell damage and activity reduction induced by C. tropicalis adhesion and the role of SAPT gene e...

Nanotechnology applied to medical biofilms control

Nanoscience and Nanotechnology (N&N) are new approaches to research phenomena at atomic, molecular and macromolecular scales, where properties differ significantly from those at a larger scale. This new approach can be applied to microbial biofilms, which are formed when bacterial and/or yeast cells adhere to abiotic and biotic surfaces. It is well known that microorganisms in biofilms have a different behaviou...

Adhesion of non-Candida albicans Candida spp to urinary epithelial cells

Non-Candida albicans Candida (NCAC) spp, are nowadays responsible for more than 50% of Candida infections and it has been shown that the most prevalent NCAC spp are C.tropicalis, C. parapsilosis and C. glabrata. An important step on NCAC spp infection is their ability to adhere to the host tissues. Therefore, the aim of this study was to evaluate the ability of C.tropicalis, C. parapsilosis and C. glabrata to a...

Candida tropicalis biofilms on catheters : formation and effect on urinary epit...