Encontrados 4 documentos, a visualizar página 1 de 1
Reverse transcription-polymerase chain reaction construction of plasmid-based, ...
Couvreur,Bernard; Bollen,Alex; Le Ray,Dominique; Dujardin,Jean-ClaudeWe describe a streamlined reverse transcription-polymerase chain reaction methodology for constructing full-length cDNA libraries of trypanosomatids on the basis of conserved sequences located at the 5' and 3'ends of trans-spliced mRNAs. The amplified cDNA corresponded to full-length messengers and was amenable to in vitro expression. Fractionated libraries could be rapidly constructed in a plasmid vector by th...
Identification of an elicitin gene cluster in Phytophthora cinnamomi
Duclos, J.; Fauconnier, A.; Coelho, A. C.; Bollen, Alex; Cravador, A.; Godfroid, EdmondElicitins are a group of highly conserved proteins secreted by species of Phytophthora and a species of the related genus Pythium, Pythium vexans. Some of these proteins act as inducers of the necrotic hypersensitive-like response and the associated systemic acquired resistance phenomenon, in some species. We cloned and characterised the cinnamomin-beta and -alpha genes and two related elicitin genes from Phyto...
Identification of an elicitin gene cluster in Phytophthora cinnamomi and analys...
Duclos, J.; Aurélio, M.; Graca, J.; Coelho, A. C.; Fauconnier, A.; Jacquet, Alain; Bollen, Alex; Cravador, A.; Biemans, R.; Godfroid, EdmondThe oomycetous fungus Phytophthora cinnamoni plays and important role in the necrotic activity observed on feeder root of cork oaks (Quercus suber L.) and eucalyptus (eucalyptus marginata Donn. Ex. Sm.)trees reducing the capacity of these hosts to absorb water and nutrients.
Highly specific and sensitive non-radioactive molecular identification of Phyto...
Coelho, A. C.; Cravador, A.; Bollen, Alex; Ferraz, J. F. P.; Moreira, A. C.; Fauconnier, A.; Godfroid, EdmondIn response to the need for a faster, more reliable method for identifying Phytophthora cinnamomi in cork oak soils in Portugal, a simple, fast, sensitive molecular identification method is described. It is based on a colorimetric assay which involves an oligonucleotide capture probe covalently immobilised on microtitration wells, a multi-biotinylated oligonucleotide detection probe and the PCR-amplified target...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||