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Possibilities for recycling cellulases after use in celllase processing - part ...

Azevedo, Helena S.; Bishop, David; Paulo, Artur Cavaco

Preliminary recycling experiments with cellulase enzymes after cotton treatments at 50°C showed that activity remaining in the treatment liquors was reduced by about 80% after five recycling steps. The potential problems of end-product inhibition, thermal and mechanical deactivation, and the loss of some components of the cellulase complex by preferential and or irreversible adsorption to cotton substrates were...


Possibilities for recycling cellulases after use in cotton processing

Azevedo, Helena S.; Bishop, David; Paulo, Artur Cavaco


Possibilities for recycling cellulases after use in cotton processing - part II...

Azevedo, Helena S.; Bishop, David; Paulo, Artur Cavaco

The adsorption and activity of a total cellulase (Trichoderma reesei) was measured and compared on undyed and dyed cotton fabrics. Recovery of enzymes from the reaction mixture and by desorption from the cotton substrate was evaluated. About 80% of the initial protein could be recovered. The removal of released products (soluble reducing sugars and dyes) from the treatment liquor and subsequent concentration of...


Possibilities for recycling cellulases after use in cotton Processing. Part I :...

Azevedo, Helena S.; Bishop, David; Paulo, Artur Cavaco

Preliminary recycling experiments with cellulase enzymes after cotton treatments at 50°C showed that activity remaining in the treatment liquors was reduced by about 80% after five recycling steps. The potential problems of end-product inhibition, thermal and mechanical deactivation, and the loss of some components of the cellulase complex by preferential and or irreversible adsorption to cotton substrates were...





Effects of agitation and endoglucanase pretreatment on the hydrolysis of cotton...

Paulo, Artur Cavaco; Almeida, Luís de; Bishop, David

We have attempted to clarify the effects of low and high levels of mechanical agitation on the cellulolytic activity of a pure endoglucanase (EG) and a total cellulase mixture (TC) on scoured and bleached cotton fabric, along with the effect of EG pretreatment on subsequent treatments with TC. Methods used to follow the progress of fabric treatments include weight loss, breaking load loss, bending hysteresis, a...


Cellulase activities and finishing effects

Paulo, Artur Cavaco; Almeida, Luís de; Bishop, David

Cellulase enzymes are usually characterized by evaluation of their activities towards different forms of cellulose and their derivatives. For textile applications, it was suggested thet the comparative evaluation should also include the measurement of the activities in tests that simulate the large scale treatments with different degrees of mechanical agitation. Comparisons were made between three cellulase mix...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia