Encontrados 10 documentos, a visualizar página 1 de 1

Chitosan – poly(butylene succinate) scaffolds and human bone marrow stromal cel...

Tissue engineering sustains the need of a three-dimensional (3D) scaffold to promote the regeneration of tissues in volume. Usually, scaffolds are seeded with an adequate cell population, allowing their growth and maturation upon implantation in vivo. Previous studies obtained by our group evidenced significant growth patterns and osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) w...

Influence of conditioned medium over the chondrogenic differentiation of adult ...

Chitosan/polyester-based scaffolds for cartilage tissue engineering : assessmen...

Naturally derived polymers have been extensively used in scaffold production for cartilage tissue engineering. The present work aims to evaluate and characterize extracellular matrix (ECM) formation in two types of chitosan-based scaffolds, using bovine articular chondrocytes (BACs). The influence of these scaffolds’ porosity, as well as pore size and geometry, on the formation of cartilagineous tissue was stud...

Melt-based compression-molded scaffolds from chitosan-polyester blends and comp...

Blends of chitosan and synthetic aliphatic polyesters (polybutylene succinate, polybutylene succinate adipate, polycaprolactone, and polybutylene terepthalate adipate) were compounded with and without hydroxyapatite, a bioactive mineral filler known to enhance osteoconduction. The blends and composites were compression molded with two different granulometric salt sizes (63–125 lm and 250–500 lm) having differen...

Osteogenic differentiation of human bone marrow mesenchymal stem cells seeded o...

The purpose of this study was to evaluate the growth patterns and osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) when seeded onto new biodegradable chitosan/polyester scaffolds. Scaffolds were obtained by melt blending chitosan with poly(butylene succinate) in a proportion of 50% (wt) each and further used to produce a fiber mesh scaffold. hBMSCs were seeded on those structures ...

Adhesion, proliferation, and osteogenic differentiation of a mouse mesenchymal ...

The aim of the present work was to study the biological behavior of a mouse mesenchymal stem cell line when seeded and cultured under osteogenic conditions onto novel processed melt-based chitosan scaffolds. Scaffolds were produced by compression molding, followed by salt leaching. Scanning electron microscopy (SEM) observations and lCT analysis showed the pore sizes ranging between 250 and 500 lm and the inter...

Assessment of the suitability of chitosan/polybutylene succinate scaffolds seed...

In this work, scaffolds derived from a new biomaterial originated from the combination of a natural material and a synthetic material were tested for assessing their suitability for cartilage tissue engineering applications. In order to obtain a better outcome result in terms of scaffolds’ overall properties, different blends of natural and synthetic materials were created. Chitosan and polybutylene succinate (...

Water absorption and degradation characteristics of chitosan-based polyesters a...

Blends of chitosan and biodegradable synthetic aliphatic polyesters (polycaprolactone, poly-(butylene succinate), poly[(butylene succinate)-co-adipate], poly[(butylene terephthalate)-co-adipate], and poly(lactic acid)) were injection-molded. These samples were immersed in isotonic solution at 37ºC for a period of 60 d. The water uptake and the degradation properties, as measured by the loss in tensile strength,...

Hydroxyapatite reinforced chitosan and polyester blends for biomedical applicat...

Hydroxyapatite, chitosan, and aliphatic polyester were compounded using a twin-screw extruder. The polyesters include poly(e-caprolactone) (PCL), poly(lactic acid) , poly(butylene succinate) (PBS), and poly(butylene terephthalate adipate). The mass fraction of chitosan ranged from 17.5 to 45%, while that of HA ranged from 10 to 30%. These blends were injection molded and evaluated for thermal, morphological, an...

Properties of melt processed chitosan and aliphatic polyester blends

The activities (at pH 7 and 50°C) of purified EGV (Humicola insolens) and CenA (Cellulomonas fimi) were determined on cotton fabrics at high and low levels of mechanical agitation. Similar activity measurements were also made by using the core domains of these cellulases. Activity experiments suggested that the presence of cellulose binding domains (CBDs) is not essential for cellulase performance in the textil...