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Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH
Almeida, Carina; Azevedo, N. F.; Bento, Joana C.; Cerca, Nuno; Ramos, H.; Vieira, M. J.; Keevil, C. W.We developed a fluorescence in situ hybridization (FISH) method for the rapid detection of Proteus spp. in urine, using a novel peptide nucleic acid (PNA) probe. Testing on 137 urine samples from patients with urinary tract infections has shown specificity and sensitivity values of 98 % (95 % CI, 93.2–99.7) and 100 % (95 % CI, 80,8–100), respectively, when compared with CHROMagar Orientation medium. Results ind...
Farnesol induces cell detachment from established S. epidermidis biofilms
Cerca, Nuno; Gomes, F. I.; Bento, Joana C.; França, Ângela; Rolo, Joana; Miragaia, Maria; Teixeira, P.; Oliveira, RosárioAntibiotic resistance is a serious problem in Staphylococcus epidermidis infections as many clinical isolates of this organism are resistant to up to eight different antibiotics. The increased resistance to conventional antibiotic therapy has lead to the search for new antimicrobial therapeutic agents. Farnesol, an essential oil found in many plants, has been shown to be active against S. epidermidis. Using a t...
Variability of RNA quality extracted from biofilms of foodborne pathogens using...
França, Ângela; Bento, Joana C.; Cerca, NunoThe biofilm formation by foodborne pathogens is known to increase the problem related with surface disinfection procedure in the food processing environment and consequent transmission of these pathogens into the population. Messenger RNA has been increasingly used to understand the action and the consequences of disinfectants in the virulence on such biofilms. RNA quality is an important requirement for any RN...
Farnesol antimicrobial role as biofilm cell detachment inducer in S. epidermidi...
Cerca, Nuno; Gomes, F. I.; França, Ângela; Bento, Joana C.; Rolo, Joana; Miragaia, Maria; Teixeira, P.; Vilanova, Manuel; Oliveira, RosárioObjetives: Farnesol is a naturally-occurring sesquiterpene that was originally isolated from essential oils found in many plants has been described to have antimicrobial potential against several bacteria, including S. epidermidis. However, farnesol mechanism of action is not yet fully understood and some contradictory findings have been reported. We recently described that while farnesol was not efficient at k...
Development of a rapid method for Proteus spp. detection in urine by peptide nu...
Almeida, Carina; Azevedo, N. F.; Cerqueira, L.; Bento, Joana C.; Vieira, M. J.; Keevil, C. W.The need to avoid empirical treatment of patients, and the fact that urine samples are among the most numerous of specimen types sent for microbiology studies, have prompted many researchers to explore methods to limit the time and expense of urine culture processing. The aim of this work was to develop a new peptide nucleic acid fluorescence in situ hybridization (PNA FISH) method for the rapid detection of Pr...
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