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Futile cycling of lactate through the plasma membrane of C6 glioma cells as det...
Rodrigues, Tiago B.; Gray, Heather L.; Benito, Marina; Garrido, Susana; Sierra, Alejandra; Geraldes, Carlos F.; Ballesteros, Paloma; Cerdán, SebastiánWe report a novel (13C, 2H) nuclear magnetic resonance (NMR) procedure to investigate lactate recycling through the monocarboxylate transporter of the plasma membrane of cells in culture. C6 glioma cells were incubated with [3-13C]lactate in Krebs-Henseleit Buffer containing 50% 2H2O (vol/vol) for up to 30 hr. 13C NMR analysis of aliquots progressively taken from the medium, showed: (1) a linearly decreasing si...
Data: 2005
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Origem: Estudo Geral - Universidade de Coimbra
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