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Cloning, characterization and in vitro and in planta expression of a glucanase ...

Oomycetes from the genus Phytophthora are fungus-like plant pathogens that are devastating for agriculture and natural ecosystems. They are able to secrete a glucanase inhibitor protein (GIP) that inhibits the activity of endoglucanases (EGases) involved in defense responses against infection. One of the most widely distributed and aggressive Phytophthora species, with more than 1,000 host plants is P. cinnamom...

Isolation and analysis of lip2 gene from Trichoderma harzianum

PTDC/AGR-AAM/67628/2006 - dentification, characterization and role of molecular factors associated with the mechanisms of infection of Fagaceae species by Phytophthora cinnamomi

Isolation and analysis of lip2 gene from Trichoderma harzianum

PTDC/AGR-AAM/67628/2006 - Identification, characterization and role of molecular factors associated with the mechanisms of infection of Fagaceae species by Phytophthora cinnamomi ; Trichodorma spp. covers a group of fungi extremely common in soils of all climatic areas. These fungi arc efficient enzyme producers, with industrial applications, or in nature, involved in the degradation of the coli wall of the ph...

Isolation and characterization of necrosis-inducing phytophthora protein 1 (npp...

Oomycetes from the genus Phytophthora are fungus-like plant pathogens that are devastating for agriculture and natural ecosystems. Due to their particular physiological characteristics, no efficient treatments against diseases caused by these microorganisms are presently available. To develop such treatments, it appears essential to dissect the molecular mechanisms that determine the interaction between Phytoph...

Characterization of a glucanase inhibitor protein from Phytophthora cinnamomi i...

Oomycetes from the genus Phytophthora are fungos-likeplant pathogens that are devastating fr agriculture and natural ecosystems. Due to their partucular physiological characteristics, no eficiente tratmentts, it against diseases caused by these microorganisms are presently available. To develop such treatments, it appears essential to dissect the molecular mechanisms that determine the interaction between Phy...

Expression analysis by RT-qPCR of NPP1 gene from Phytophthora cinnamomi

Expression analysis by RT-qPCR of NPP1 gene from Phytophthora cinnamomi

Species of the genus Phytophthora secrete glucanase inhibitor proteins (GIPs ) to inhibit the activity of enzymes involved in plant defense responses, including during plant infection process of Castanea sativa by Phytophthora cinnamomi. GIPs show structural homology to the chymotrypsin class of serine proteases (SP) but lack proteolytic activity due to the absence of an intact catalytic triad and, thus, belong...

Expression analysis by RT-PCR of GIP gene from Phytophthora cinnamomi

Expression analysis by RT-PCR of GIP gene from Phytophthora cinnamomi

Species of the genus Phytophthora secrete glucanase inhibitor proteins (GIPs) to inhibit the activity of enzymes involved in plant defense responses, including during plant infection process of Castanea sativa Mill by Phytophthora cinnamomi. GIPs show structural homology to the chymotrypsin class of serine proteases (SP) but lack proteolytic activity due to the absence of an intact catalytic triad and, thus, be...

Characterization of transglutaminase elicitor precursor from plants pathogen P...

The oomycetes form a phylogenetically distinct group of eukaryotic microorganisms that includes some of the most notorious pathogens of plants. Among these, members of the genus Phytophthora cause enormous economic losses on crop species as well as environmental damage in natural ecosystems. Phytophthora cinnamomi is the most widely distributed Phytophthora species, with nearly 1000 host species.