Encontrados 12 documentos, a visualizar página 1 de 2
Heat and water stress induce unique transcriptional signatures of heat-shock pr...
Rocheta, M.; Becker, J.D.; Coito, J.L.; Carvalho, L.; Amâncio, S.Grapevine is an extremely important crop worldwide. In southern Europe, post-flowering phases of the growth cycle can occur under high temperatures, excessive light, and drought conditions at soil and/or atmospheric level. In this study, we subjected greenhouse grown grapevine, variety Aragonez, to two individual abiotic stresses, water deficit stress (WDS), and heat stress (HS). The adaptation of plants to str...
A genome-wide survey of sRNAs in the symbiotic nitrogen-fixing alpha-proteobact...
Schluter, J.P.; Reinkensmeier, J.; Daschkey, S.; Evguenieva-Hackenberg, E.; Janssen, S.; Janicke, S.; Becker, J.D.; Giegerich, R.; Becker, A.A total of 1,125 sRNA candidates that were classified as trans-encoded sRNAs (173), cis-encoded antisense sRNAs (117), mRNA leader transcripts (379), and sense sRNAs overlapping coding regions (456) were identified in a size range of 50 to 348 nucleotides. Among these were transcripts corresponding to 82 previously reported sRNA candidates. Enrichment for RNAs with primary 5'-ends prior to sequencing of cDNAs s...
Epigenetic reprogramming and small RNA silencing of transposable elements in po...
Slotkin, R.K.; Vaughn, M.; Borges, F.; Tanurdzic, M.; Becker, J.D.; Feijó, J.A.; Martienssen, R.The mutagenic activity of transposable elements (TEs) is suppressed by epigenetic silencing and small interfering RNAs (siRNAs), especially in gametes that could transmit transposed elements to the next generation. In pollen from the model plant Arabidopsis, we show that TEs are unexpectedly reactivated and transpose, but only in the pollen vegetative nucleus, which accompanies the sperm cells but does not prov...
Overexpression of delta-like 4 induces arterialization and attenuates
Trindade, A.; Kumar, S.R.; Scehnet, J.S.; Lopes-da-Costa, L.; Becker, J.D.The importance of Notch signaling pathway in the regulation of vascular development and angiogenesis is suggested by the expression of Notch receptors and ligands in vascular endothelial cells (ECs) and the observed vascular phenotypes in mutants of Notch receptors or ligands, especially Dll4. DLL4 is specifically expressed in arterial ECs during development, and haplo-insufficiency is embryonically lethal in m...
Genetic subtraction profiling identifies genes essential for Arabidopsis reprod...
Johnston, A.J.; Meier, P.; Gheyselinck, J.; Federer, M.; Wuest, A.E.J.; Schlagenhauf, E.; Becker, J.D.; Grossnikalus, U.The embryo sac contains the haploid maternal cell types necessary for double fertilization and subsequent seed development in plants. Large-scale identification of genes expressed in the embryo sac remains cumbersome because of its inherent microscopic and inaccessible nature. We used genetic subtraction and comparative profiling by microarray between the Arabidopsis thaliana wild-type and a sporophytic mutant ...
Cyclooxygenase-2 (COX-2) expression by in vitro produced bovine embryos. Prelim...
Marques, C.C.; Horta, A.E.; Vasques, M.I.; Baptista, M.C.; Becker, J.D.; Pimenta, J.; Pereira, R.M.Expression of the two isoforms of cyclooxygenase enzyme, cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2), by in vitro produced bovine embryos was examined. Cumulus-oocyte complexes were recovered from ovaries of slaughtered animals and subsequently in vitro matured and in vitro fertilized. Presumptive zygotes were cultured in serum containing medium (TCM 199+10% bovine superovulated oestrus serum) on a gr...
Gametophyte interaction and sexual reproduction: how plants make a zygote
Boavida, L.C.; Vieira, A.M.; Becker, J.D.; Feijó, J.A.The evolutionary success of higher plants relies on a very short gametophytic phase, which underlies the sexual reproduction cycle. Sexual plant reproduction takes place in special organs of the flower: pollen, the male gametophyte, is released from the anthers and then adheres, grows and interacts along various tissues of the female organs, collectively known as the pistil. Finally, it fertilizes the female ga...
Gene family analysis of the Arabidopsis pollen transcriptome reveals biological...
Pina, C.; Pinto, F.; Feijó, J.A.; Becker, J.D.Upon germination, pollen forms a tube that elongates dramatically through female tissues to reach and fertilize ovules. While essential for the life cycle of higher plants, the genetic basis underlying most of the process is not well understood. We previously used a combination of flow cytometry sorting of viable hydrated pollen grains and GeneChip array analysis of onethird of the Arabidopsis (Arabidopsis thal...
The making of gametes in higher plants
Boavida, L.; Becker, J.D.; Feijó, J.A.Higher plants have evolved to be one of the predominant life forms on this planet. A great deal of this evolutionary success relies in a very short gametophytic phase which underlies the sexual reproduction cycle. Sexual plant reproduction takes place in special organs of the flower. In most species the processes of gametogenesis, pollination, syngamy and embryogenesis are sequentially coordinated to give rise ...
Signalling by tips
Feijó, J.A.; Costa, S.S.; Prado, A.M.; Becker, J.D.; Certal, A.C.New molecules, including protein kinases, lipids and molecules that have neurotransmitter activities in animals have emerged as important players in tip-growing cells. Transcriptomics analysis reveals that the largest single class of genes expressed in pollen tubes encode signal transducers, reflecting the necessity to decode complex and diverse pathways that are associated with tip growth. Many of these pathwa...
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