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Flow cytometry for age assessment of a yeast population and its application in ...
Kuřec, Michal; Baszczyňski, Martin; Lehnert, Radek; Mota, André; Teixeira, J. A.; Brányik, TomášAn expeditious method of yeast age estimation was developed based on selective bud scar staining (Alexa Fluor 488-labelled wheat-germ agglutinin) and subsequent fluorescence intensity measurement by flow cytometry. The calibration curve resulting from the cytometric determination of average bud scar fluorescence intensities vs. microscopically counted average bud scar numbers of the same cell populations showed...
Data: 2009
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Origem: RepositóriUM - Universidade do Minho
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