Encontrados 5 documentos, a visualizar página 1 de 1
DNA-based biosensor for the electrocatalytic determination of antioxidant capac...
Barroso, M. Fátima; de-los-Santos-Álvarez, N.; Lobo-Castañón, M. J.; Miranda-Ordieres, A. J.; Delerue-Matos, Cristina; Oliveira, M. Beatriz P. P.Reactive oxygen species (ROS) are produced as a consequence of normal aerobic metabolism and are able to induce DNA oxidative damage. At the cellular level, the evaluation of the protective effect of antioxidants can be achieved by examining the integrity of the DNA nucleobases using electrochemical techniques. Herein, the use of an adenine-rich oligonucleotide (dA21) adsorbed on carbon paste electrodes for the...
Towards a reliable technology for antioxidant capacity and oxidative damage eva...
Barroso, M. Fátima; de-los-Santos-Álvarez, N.; Delerue-Matos, Cristina; Oliveira, M. Beatriz P. P.To counteract and prevent the deleterious effect of free radicals the living organisms have developed complex endogenous and exogenous antioxidant systems. Several analytical methodologies have been proposed in order to quantify antioxidants in food, beverages and biological fluids. This paper revises the electroanalytical approaches developed for the assessment of the total or individual antioxidant capacity. ...
Gold electrode modified by self-assembled monolayers of thiols to determine DNA...
Silva, Mízia M. S.; Cavalcanti, Igor T.; Barroso, M. Fátima; Sales, M. Goreti F.; Dutra, Rosa FiremanThe process of immobilization of biological molecules is one of the most important steps in the construction of a biosensor. In the case of DNA, the way it exposes its bases can result in electrochemical signals to acceptable levels. The use of self-assembled monolayer that allows a connection to the gold thiol group and DNA binding to an aldehydic ligand resulted in the possibility of determining DNA hybridiza...
Survey of trace elements (Al, As, Cd, Cr, Co, Hg, Mn, Ni, Pb, Se, and Si) in re...
Barroso, M. Fátima; Ramos, Sandra; Oliva-Teles, Maria Teresa; Delerue-Matos, Cristina; Sales, M. Goreti F.; Oliveira, M. Beatriz P. P.Concentrations of eleven trace elements (Al, As, Cd, Cr, Co, Hg, Mn, Ni, Pb, Se, and Si) were measured in 39 (natural and flavoured) water samples. Determinations were performed using graphite furnace electrothermetry for almost all elements (Al, As, Cd, Cr, Co, Mn, Ni, Pb, and Si). For Se determination hydride generation was used, and cold vapour generation for Hg. These techniques were coupled to atomic absor...
Flow amperometric determination of carbofuran and fenobucarb
Sales, M. Goreti F.; Vaz, M. Carmo V. F.; Delerue-Matos, Cristina; Almeida, Sofia A. A.; Barroso, M. Fátima; Ferreira, Helder A. O.A simple, rapid, and precise amperometric method for quantification of N-methylcarbamate pesticides in water samples and phytopharmaceuticals is presented. Carbofuran and fenobucarb are the target analytes. The method is developed in flow conditions providing the anodic oxidation of phenolic-based compounds formed after alkaline hydrolysis. Optimization of instrumental and chemical variables is presented. Under...
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