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A kinetic model for hydrolysis of whey proteins by cardosin a extracted from Cy...
Barros, Rui M.; Malcata, F. XavierThe enzymatic hydrolysis of the major whey proteins, namely b-lactoglobulin (b-Lg) and a-lactalbumin (a-La), was experimentally studied using whey as substrate; an aspartic protease (cardosin A), previously extracted from the flowers of Cynara cardunculus and purified by gel filtration and ion exchange chromatographies, was used for this purpose. Sweet whey was incubated for 24 h at various enzyme:substrate rat...
Hydrolysis of α-lactalbumin by cardosin A immobilized on highly activated supports
Barros, Rui M.; Extremina, Clara I.; Gonçalves, Inês C.; Braga, Beatriz O.; Balcão, Victor M.; Malcata, F. XavierIn the present research effort, production of derivatives of cardosin A (a plant protease) encompassing full stabilization of its dimeric structure has been achieved, via covalent, multi-subunit immobilization onto highly activated agarose-glutaraldehyde supports. Boiling such enzyme derivatives in the presence of sodium dodecyl sulfate and β-mercaptoethanol did not lead to leaching of enzyme, thus providing ev...
Quantitative studies on the enzymatic hydrolysis of milk proteins brought about...
Barros, Rui M.; Ferreira, Carla A. Ferreira; Silva, Sofia V. Silva; Malcata, F. XavierHydrolysis of whey proteins may produce peptide mixtures with better functional properties than the original protein mixture, viz. higher solubilites and lower allergenic effects. Cynara cardunculus is a wild plant that possesses (aspartic) proteases in its flower cells; those enzymes exhibit general proteolytic and specific milk clotting activities, which are rather useful in traditional cheesemaking. This stu...
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