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mRNA-Seq and microarray development for the Grooved carpet shell clam, Ruditape...

Leite, Ricardo B.; Milan, Massimo; Coppe, Alessandro; Bortoluzzi, Stefania; dos Anjos, António; Reinhardt, Richard; Saavedra, Carlos; Patarnello, T.

Background: The Grooved Carpet shell clam Ruditapes decussatus is the autochthonous European clam and the most appreciated from a gastronomic and economic point of view. The production is in decline due to several factors such as Perkinsiosis and habitat invasion and competition by the introduced exotic species, the manila clam Ruditapes philippinarum. After we sequenced R. decussatus transcriptome we have desi...


Global analysis of gene expression in mineralizing fish vertebra-derived cell l...

Tiago, Daniel M.; Laizé, Vincent; Bargelloni, Luca; Ferraresso, Serena; Romualdi, Chiara; Cancela, Leonor

Background Fish has been deemed suitable to study the complex mechanisms of vertebrate skeletogenesis and gilthead seabream (Sparus aurata), a marine teleost with acellular bone, has been successfully used in recent years to study the function and regulation of bone and cartilage related genes during development and in adult animals. Tools recently developed for gilthead seabream, e.g. mineralogenic cell lines ...


Transcriptome sequencing and microarray development for the Manila clam, Rudita...

Milan, Massimo; Coppe, Alessandro; Reinhardt, Richard; Cancela, Leonor; Leite, Ricardo B.; Saavedra, Carlos; Ciofi, Claudio; Chelazzi, Guido

Abstract Background The Manila clam, Ruditapes philippinarum, is one of the major aquaculture species in the world and a potential sentinel organism for monitoring the status of marine ecosystems. However, genomic resources for R. philippinarum are still extremely limited. Global analysis of gene expression profiles is increasingly used to evaluate the biological effects of various environmental stressors on aq...


Global analysis of gene expression in mineralizing fish vertebra-derived cell l...

Tiago, Daniel M.; Laizé, Vincent; Bargelloni, Luca; Ferraresso, Serena; Romualdi, Chiara; Cancela, Leonor

Abstract Background Fish has been deemed suitable to study the complex mechanisms of vertebrate skeletogenesis and gilthead seabream (Sparus aurata), a marine teleost with acellular bone, has been successfully used in recent years to study the function and regulation of bone and cartilage related genes during development and in adult animals. Tools recently developed for gilthead seabream, e.g. mineralogenic ce...


Skin healing and scale regeneration in fed and unfed sea bream, Sparus auratus

Vieira, Florbela A.; Gregório, Sílvia F.; Ferraresso, Serena; Thorne, Michael A. S.; Costa, Rita; Milan, Massimo; Bargelloni, Luca; Clark, M. S.

Abstract Background Fish scales are an important reservoir of calcium and phosphorus and together with the skin function as an integrated barrier against environmental changes and external aggressors. Histological studies have revealed that the skin and scales regenerate rapidly in fish when they are lost or damaged. In the present manuscript the histological and molecular changes underlying skin and scale rege...


Development and validation of a gene expression oligo microarray for the gilthe...

Ferraresso, Serena; Vitulo, Nicola; Mininni, Alba N.; Romualdi, Chiara; Cardazzo, Barbara; Negrisolo, Enrico; Reinhardt, Richard; Canario, Adelino V. M.

Aquaculture represents the most sustainable alternative of seafood supply to substitute for the declining marine fisheries, but severe production bottlenecks remain to be solved. The application of genomic technologies offers much promise to rapidly increase our knowledge on biological processes in farmed species and overcome such bottlenecks. Here we present an integrated platform for mRNA expression profiling...


A gene-based radiation hybrid map of the gilthead sea bream Sparus aurata refin...

Sarropoulou, Elena; Franch, Rafaella; Louro, Bruno; Power, Deborah; Bargelloni, Luca; Magoulas, Antonios; Senger, Fabrice; Kotoulas, Georgios

Background: Comparative teleost studies are of great interest since they are important in aquaculture and in evolutionary issues. Comparing genomes of fully sequenced model fish species with those of farmed fish species through comparative mapping offers shortcuts for quantitative trait loci (QTL) detections and for studying genome evolution through the identification of regions of conserved synteny in teleosts...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia