Esta dissertação aborda a implementação de um sistema de gestão de iluminação especialmente concebido para operar em ambientes industriais, assumindo-se como uma solução respeitadora do ambiente e preocupada com o conforto global de todos os seus funcionários. O sistema foi construído com uma topologia mestre/ escravo, a fim de dar ao utilizador a necessária interatividade. Tem a possibilidade de ser utilizado ...
Doutoramento em Engenharia Alimentar - Instituto Superior de Agronomia ; The grape berry microbiota is deeply influenced by the sanitary status of grapes. In sound grapes the most frequent yeast species were basidiomycetes, Aureobasidium pullulans, Candida zemplinina and Hanseniaspora uvarum. Sour rotten grapes were mostly colonised by C. zemplinina, H. uvarum, Issatchenkia spp. and Zygoascus hellenicus. The p...
The aim of this work was to determine the ability of six yeast and two bacterial species associated with wine spoilage to form biofilms in mono- or co-culture using the Calgary Biofilm Device (CBD). Moreover, the efficacy of several disinfectants was evaluated against these spoilage microorganisms, both in the planktonic and the biofilm states. Results showed that Dekkera bruxellensis, Saccharomyces cerevisiae,...
Com o aumento de utilização de sistemas de informação geográfica na internet, surge a distribuição de informação georreferenciada e procedimentos para a sua manipulação, estando o acesso a esta informação disponível em vários tipos de dispositivos, a partir de qualquer localização e a qualquer utilizador. Tipicamente, a informação geográfica é obtida e tratada por empresas especializadas. O papel dos utilizador...
The wine spoilage yeast Dekkera bruxellensis was evaluated for the production of 4-ethylphenol under low concentrations (0.02–20 g L 1) of glucose and fructose in synthetic media. Measurable amounts of 4-ethylphenol were produced over 0.2 g L 1 of each sugar. The yeast growth rate and amount of biomass formed increased from 0.2 to 20 g L 1 of glucose or fructose, being accompanied by increasing production of 4-...
Minireview ; Yeast species of sound and sour rot-damaged grapes were analysed during fermentation and grape ripening in the vineyard, using general and selective culture media. During 2003 and 2004 vintages, microvinifications were carried out with sound grapes to which different amounts of grapes with sour rot were added. The wine spoilage species Zygosaccharomyces bailii was only recovered during fermentatio...
The wine spoilage yeast species Dekkera bruxellensis, after inoculation in red wines, displayed three survival patterns characterized by: i) initial lag phase followed by growth and sequential death; ii) initial death phase leading to reduced viable counts followed by growth and sequential death; and iii) death phase leading to complete loss of viability. These survival patterns were observed for the same strai...
Several microbial species associated with wine were challenged against increasing concentrations of dimethyl dicarbonate (DMDC). The concentration inducing complete cell death upon addition to red wine was regarded as the minimum inhibitory concentration (MIC). In dry red wines with 12% (v/v) ethanol and pH 3.50, the inactivation depended on the initial cell concentration. For an initial inoculum of 500 CFU/ml,...
Aims: To identify ascomycetous yeasts recovered from sound and damaged grapes by the presence of honeydew or sour rot. Methods and Results: In sound grapes, the mean yeast counts ranged from 3.20 ± 1.04 log CFU g-1 to 5.87 ± 0.64 log CFU g-1. In honeydew grapes, the mean counts ranged from 3.88 ± 0.80 log CFU g-1 to 6.64 ± 0.77 log CFU g-1. In sour rot grapes counts varied between 6.34 ± 1.03 and 7.68 ± 0.38 lo...
A total of 63 strains of Dekkera bruxellensis and 32 strains of Pichia guilliermondii isolated from wine related environments were identified by restriction analysis of the 5.8S-ITS region of the rDNA. These strains were subjected to intraspecific discrimination using mtDNA restriction and RAPD-PCR analysis. The isolates identified as D. bruxellensis yielded 3 different molecular patterns of mtDNA restriction u...
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