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Structural and functional stabilization of L-asparaginase via multisubunit immo...
Balcão, Vitor M.; Mateo, Cesar; Fernández-Lafuente, R.; Malcata, F. Xavier; Guisán, José M.A new protocol for the stabilization of the quaternary structure of multimeric enzymes has been attempted using as model enzyme (tetrameric) L-asparaginase from Escherichia coli. Such strategy is based upon multisubunit covalent immobilization of the enzyme onto activated supports (agarose-glutaraldehyde). Supports activated with different densities of reactive groups were used; the higher the density of groups...
Bioreactors with immobilized lipases: state of the art
Balcão, Vitor M.; Paiva, Ana L.; Malcata, F. XavierThis review attempts to provide an updated compilation of studies reported in the literature pertaining to reactors containing lipases in immobilizedforms, in a way that helps the reader direct a bibliographic search and develop an integrated perspective of the subject. Highlights are given to industrial applications of lipases (including control and economic considerations), as well as to methods of immobiliza...
Adsorption of protein from several commercial lipase preparations onto a hollow...
Balcão, Vitor M.; Vieira, Madalena C.; Malcata, F. XavierTen commercially available crude preparations of lipase from various microbial sources were adsorbed from aqueous buffers at several initial concentrations onto a bundle of hydrophobic hollow fibers made of poly(propylene) at pH 7.0 and 40 °C. The kinetics of adsorption were evaluated from measurements at various times of the protein content of the supernatant solution (using BSA as equivalent) in a well-mixed ...
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