BACKGROUND: The pinewood nematode (PWN) Bursaphelenchus xylophilus is an important conifer disease worldwide. It is the direct cause of the death of millions of pines in south-east Asia (mainly Japan, China and Korea) and has been established in Portugal since 1999. The phasing out of methyl bromide has created an urgent need for alternative treatment of wood packagingmaterials. The effect of sulfuryl fluoride ...
The Portuguese pine forest has become dangerously threatened by pine wilt disease (PWD), caused by the pinewood nematode (PWN), Bursaphelenchus xylophilus. Synthetic chemicals are the most common pesticides used against phytoparasitic nematodes but its use has negative ecological impacts. Phytochemicals may prove to be environmentally friendly alternatives. Essential oils (EOs) and decoction waters, isolated fr...
Abstract Pine wilt disease, caused by the nematode Bursaphelenchus xylophilus, is responsible for devastation of pine forests worldwide. Until now, there are no effective ways of dealing with this serious threat. The use of 1-aminocyclopropane-1-carboxylate (ACC) deaminase (encoded by the acdS gene)-producing plant growth-promoting bacteria has been shown to be a useful strategy to reduce the damage due to biot...
Mestrado em Psicologia Social e das Organizações ; No leque de emoções auto-conscientes que assumem uma grande importância a nível individual e interpessoal, encontra-se a vergonha. Esta emoção que faz parte do nosso quotidiano, apresenta impacto na forma como lidamos com as situações, tornando-se assim pertinente compreender os seus fundamentos de base. Vários estudos e pesquisas focadas na vergonha têm demon...
Pine wilt disease (PWD) has a tremendous impact on worldwide forestlands, both from the environmental and economical viewpoints. Monochamus sp., a xylophagous insect from the Cerambycidae family, plays an important role in dissemination of the pinewood nematode, Bursaphelenchus xylophilus, the primary pathogenic agent of PWD. This study investigates, for the first time, the bacterial communities of Monochamus g...
Dissertação de mestrado em Bioinformática ; Over the years, metagenomics has demonstrated to play an essential role on the study of the microorganisms that live in microbial communities, particularly those who inhabit the human body. Several bioinformatic tools and pipelines have been developed, but usually they only address one question: "Who is there?" or "What are they doing?". This work aimed to develop a ...
O presente relatório, realizado no âmbito do Mestrado de Ensino em 1.º e 2.º Ciclo do Ensino Básico, descreve o trabalho desenvolvido na unidade curricular Prática de Ensino Supervisionada (PES). Esta prática decorreu no ano letivo 2012/2013, em dois contextos, contexto de 1.º Ciclo do Ensino Básico (CEB) numa turma de 3.º ano de escolaridade e no contexto de 2.º Ciclo do Ensino Básico com duas turmas de 5.º e ...
Pine wilt disease (PWD) is a complex disease integrating three major agents: the pathogenic agent, the pinewood nematode Bursaphelenchus xylophilus; the insect-vector Monochamus spp.; and the host pine tree, Pinus sp. Since the early 80's, the notion that another pathogenic agent, namely bacteria, may play a role in PWD has been gaining traction, however the role of bacteria in PWD is still unknown. The present...
The pine wood nematode (PWN), Bursaphelenchus xylophilus, the causal agent of pine wilt disease (PWD), is a major world-wide pathogen and pest of pine, with impacts on forest health, natural ecosystem stability and international trade. In Portugal, PWN was first diagnosed in 1999, the first occurrence also for Europe. The disease was recently detected on the island ofMadeira and in northern Spain. In an attempt...
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