Encontrados 11 documentos, a visualizar página 1 de 2
Odorant binding proteins : a biotechnological tool for odour control
Silva, Carla Manuela; Matamá, Maria Teresa; Azoia, Nuno G.; Mansilha, Catarina; Casal, Margarida; Paulo, Artur CavacoThe application of an odorant binding protein for odour control and fragrance delayed release from a textile surface was first explored in this work. Pig OBP-1 gene was cloned and expressed in Escherichia coli , and the purified protein was biochemically characterized. The IC50 values(concentrations of competitor that caused a decay of fluorescence to half-maximal intensity) were determined for four distinct fr...
The activity of LE10 peptide on biological membranes using molecular dynamics, ...
Antunes, Egipto; Azoia, Nuno G.; Matamá, Maria Teresa; Gomes, Andreia; Paulo, Artur CavacoCell-penetrating peptides (CPPs) and antimicrobial peptides (AMPs) are generally defined as small cationic peptides with the ability to interact with lipidic membranes, in a process driven by electrostatic and hydrophobic processes. The interaction with CPPs is known to lead to its translocation across the membrane, while with AMPs lead to membrane damage. Here we present one synthetic anionic peptide, LE10 (L...
In vitro and computational studies of transdermal perfusion of nanoformulations...
Martins, M.; Azoia, Nuno G.; Ribeiro, Artur J.; Shimanovich, Ulyana; Silva, Carla; Paulo, Artur CavacoTransdermal perfusion of a large protein is reported for the first time, using a nanoemulsion of bovine serum albumin (66 kDa) of 160 nm prepared by a solid-in-oil (S/O) process. Molecular dynamics simulations confirmed skin permeation by these formulations, with integration of the protein into the lipid bilayers. These results demonstrate the real possibility of delivering large proteins transdermally for a ra...
Keratins and lipids in ethnic hair
Cruz, Célia F.; Fernandes, Margarida M.; Gomes, Andreia; Coderch, L.; Martí, M.; Méndez, Sandra; Gales, Luís; Azoia, Nuno G.; Shimanovich, UlyanaHuman hair has an important and undeniable relevance in society due to its important role in visual appearance and social communication. Hair is mainly composed of structural proteins, mainly keratin and keratin associated proteins, and lipids. Herein, we report a comprehensive study of the content and distribution of the lipids among ethnic hair, African, Asian and Caucasian hair. More interestingly, we also r...
Molecular modeling of hair keratin/peptide complex : using MM-PBSA calculations...
Azoia, Nuno G.; Fernandes, Margarida M.; Micaelo, N. M.; Soares, Cláudio M.; Paulo, Artur CavacoMolecular dynamics simulations of a keratin/peptide complex have been conducted to predict the binding affinity of four different peptides toward human hair. Free energy calculations on the peptides' interaction with the keratin model demonstrated that electrostatic interactions are believed to be the main driving force stabilizing the complex. The molecular mechanics–Poisson-Boltzmann surface area methodology ...
Molecular recognition of esterase plays a major role on the removal of fatty so...
Silva, Carla Manuela; Azoia, Nuno G.; Martins, Madalena A.; Matamá, Maria Teresa; Wu, J.; Paulo, Artur CavacoIn this work it is describe for the first time, the use of an esterase with null activity (Tfu_0883 bacterial cutinase from Thermobifida fusca) on the removal of fat from the surface of a cotton substrate. Similar levels of fat removal were found for both null and wild-type proteins, despite that only wild type protein yielded fatty acids. Our results show that molecular recognition of esterase plays a major ro...
Insights on the mechanism of formation of protein microspheres in a biphasic sy...
Silva, Raquel; Ferreira, Helena; Azoia, Nuno G.; Shimanovich, Ulyana; Freddi, G.; Gedanken, Aharon; Paulo, Artur CavacoMicrospheres of bovine serum albumin (BSA) and silk fibroin are produced by applying ultrasound in a biphasic system consisting of an aqueous protein solution and an organic solvent. The protein microspheres are dispersed in an aqueous media where the protein remains at the interface covering the organic solvent. This only occurs when high shear forces are applied that induce changes to force the protein to the...
Tailoring elastase inhibition with synthetic peptides
Vasconcelos, Andreia; Azoia, Nuno G.; Carvalho, A. C.; Gomes, A. C.; Guebitz, G. M.; Paulo, Artur CavacoChronic wounds are the result of excessive amounts of tissue destructive proteases such as human neutrophil elastase (HNE). The high levels of this enzyme found on those types of wounds inactivate the endogenous inhibitor barrier thus, the search for new HNE inhibitors is required. This work presents two new HNE inhibitor peptides, which were synthesized based on the reactive-site loop of the Bowman–Birk inhib...
Reacções de Aza-Diels-Alder dirigidas à síntese de piperidinas polihidroxiladas...
Azoia, Nuno G.Tese de Doutoramento em Ciências - Área do Conhecimento Química ; Neste trabalho foram obtidos imino-açúcares de seis membros (piperidinas polihidroxiladas), e tetrahidroquinolinas, por reacção de cicloadição [4+2]π de 2H-azirinas/iminas com 1,3-dienos. Para a obtenção das piperidinas polihidroxiladas utilizaram-se duas metodologias diferentes. A primeira abordagem consistiu em fazer reagir 2H-azirinas com fu...
Synthesis of 1,3,8,8a-tetrahydro-3,8-epoxy[1,2-b]isoquinolines and their reacti...
Alves, M. José; Fortes, A. Gil; Azoia, Nuno G.Cycloadditions of 2H-azirines to isobenzofurans is described. The endo and exo products were obtained and were reacted with oxygen nucleophiles. Tetrahydroquinolines or benzofuranols were obtained, usually in excellent yields. Cycloaddition of the less electrophilic azirine (14) was performed at room temperature in the presence of ZnCl2. The cycloadduct was hydrolysed in the reaction conditions, but dehydration...
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