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Transcriptome of Quercus suber challenged by drought, salinity, and oxidative s...

Azevedo, Herlânder; Oliveira, Rute; Neto, T. Lino; Tavares, R. M.

Comunicação em painel ; The cork oak (Quercus suber) forest (the “montado”) is a unique and emblematic resource for Portugal, due to its ecological, socio-economic significance, and the commercial value of cork. Plant abiotic stresses, particularly reduced water availability and extreme temperatures, are substantial constraints to agricultural and agro-forestry production. In the particular case of cork oak, t...


A strategy for the identification of new abiotic stress determinants in arabido...

Azevedo, Herlânder; Correia, Joana Silva; Oliveira, Juliana Alice Ferreira; Laranjeira, Sara; Barbeta, C.; Silva, Vitor Amorim; Botella Mesa, Miguel

Since the sequencing of the Arabidopsis thaliana genome in 2000, plant researchers have faced the complex challenge of assigning function to thousands of genes. Functional discovery by in silico prediction or homology search resolved a significant number of genes, but only a minor part has been experimentally validated. Arabidopsis entry into the post-genomic era signified a massive increase in high-throughput ...


Contributions to the study of the Pinus Pinaster-Botrytis cinerea interaction

Azevedo, Herlânder

Tese de doutoramento em Ciências - Biologia ; Maritime pine (Pinus pinaster Ait.) is a two-needle pine widely distributed throughout SW Europe. P. pinaster is a pioneer species and its adaptability and phenotypical characteristics make it an economically important forest species, being considered one of the three most important pines in Europe. Botrytis cinerea is a necrotrophic fungus, requiring the killing o...


Tolerance of Pinus pinaster Ait. to salt : responses to oxidative stress

Azevedo, Herlânder; Neto, T. Lino; Tavares, R. M.

Pinus pinaster Ait. (maritime pine) is a two-needle pine distributed throughout the Western Mediterranean basin. Its characteristics make it economically important for pulp and timber industries. P. pinaster is a pioneer species thriving in dune systems, well adapted to growth in sandy silicate soils. Its increased adaptability to shoreline ecosystems should result from tolerance to salt stress, both at plant a...


An improved method for high quality RNA isolation from needles of adult maritim...

Azevedo, Herlânder; Neto, T. Lino; Tavares, R. M.

Conventional RNA isolation methods optimised for pine seedlings have been shown to produce poor quality RNA when applied to needles of adult pine trees. We describe here a modified procedure to isolate high pure RNA from needles of thirty-year-old maritime pines, exhibiting high levels of phenolics, polysaccharides and RNases. Major changes were the inclusion of proteinase K in the extraction medium followed by...


Identification of a cDNA encoding Ascorbate peroxidase from Pinus Pinaster Ait.

Correia, Joana Silva; Azevedo, Herlânder; Tavares, R. M.; Neto, T. Lino

Poster apresentado no X Congresso Nacional de Biotecnologia "BIOTEC' 2003", Lisboa, Portugal em 2003. ; The maritime or wild pine (Pinus pinaster Ait.) is the conifer with the widest distribution in Portugal, being of an extreme economic importance to wood and paper industries and also as a large source of pitch, turpentine and resin. Pine forest decay is largely associated with abiotic and biotic stresses, wh...


Molecular aproach on the study of infection of Pinus pinaster Ait. by the fungu...

Azevedo, Herlânder; Neto, T. Lino; Tavares, R. M.

Poster apresentado no 10th International Congress on Molecular Plant-Microbe Interactions, Madison, Wisconsin, Estados Unidos da América. ; The fungi genus Lophodermium comprises several species known to intervene in the degradation process of pine needles. Amongst these species, only L. seditiosum is known to be pathogenic, as it is able to infect healthy needles in young pine populations, spreading from stom...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia