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Analysis of DNA damage and repair in Saccharomyces cerevisiae using the comet a...

In this work we used the model organism Saccharomyces cerevisiae to characterise the biological activity and the mechanism of action of phytochemicals. We have assessed DNA damage and repair using the comet assay, which displayed a dose-response relationship with different DNA-damaging agents. Subsequently, we used this system to assess the antigenotoxic properties of a leaf extract from Ginkgo biloba (GBE). Ex...

Degradation of a Lithium Iron Phosphate based Cathode in Lithium-ion Batteries ...

Measuring oxidative DNA damage and DNA repair using the yeast comet assay

Chromosomal DNA damage can be a result of several processes and agents of endogenous or exogenous origin. These cause strand breaks or oxidized bases that lead to strand breaks, which relax the normally supercoiled genomic DNA and increase its electrophoretic mobility. The extent of DNA damage can be assessed by single cell gel electrophoresis, where the chromosomal DNA migration distance correlates with the ex...

Analysis of DNA damage and repair in saccharomyces cerevisiae using the comet a...

In this work we used the model organism Saccharomyces cerevisiae to characterise the biological activity and the mechanism of action of phytochemicals. One of the goals is to use mutant strains affected in basic mechanisms of oxidative stress response and DNA repair in order to uncover the molecular targets of phytochemicals. We have assessed DNA damage and repair using the comet assay, evaluated as “comet tail...

Vacuole-mitochondrial crosstalk during apoptosis in yeast: a model for understa...

The yeast apoptosis field emerged with the finding that key components of the apoptotic machinery are conserved in these simple eukaryotes. Thus it became possible to exploit these genetically tractable organisms to improve our understanding of the intricate mechanisms of cell death in higher eukaryotes and of severe human diseases associated with apoptosis dysfunctions. Early on, it was recognized that a mitoc...

MAPK signaling pathways mediate acetic acid-induced cell death in saccharomyces...

Stimulation of DNA repair in saccharomyces cerevisiae by ginkgo biloba leaf ext...

Many extracts prepared from plants traditionally used for medicinal applications contain a variety of phytochemicals with antioxidant and antigenotoxic activity. In this work we measured the DNA protective effect of extracts of Ginkgo biloba leaves from oxidative stress using Saccharomyces cerevisiae as experimental model. The extract improved viability of yeast cells under oxidative stress imposed by hydrogen ...

Sentidos associados à violência para idosos e profissionais

OBJETIVOS: Conhecer os sentidos associados à violência segundo os idosos e os profissionais. MÉTODOS: Pesquisa exploratória com abordagem qualitativa, realizada no serviço de curadoria do cidadão/delegacia especializada, na cidade de João Pessoa-PB, através de uma entrevista semiestruturada com 30 idosos e 4 profissionais que atendem o idoso vitimado. Os dados obtidos das entrevistas foram processados pelo prog...

Degradation of lithium Iron phosphate-based cathode in lithium-ion batteries: a...

Commercial Li-ion batteries were studied in view to investigate the degradation of the positive electrode in an end-of-life battery condition. Post-mortem analyses were performed by using SEM and DRX techniques; structural and morphological changes after prolonged cycling were evaluated comparatively to a fresh cathode sample. The cycling procedure based on a constant current (CC)/constant voltage (CV) charge a...

Development of an empirical correlation for Newtonian and non-Newtonian fluids ...

Most of animal and plant cell cultures present a non-Newtonian behaviour that has a deep effect on the technological performance of bioreactors, affecting the pattern of mixing, the power input required and on the mass and energy transfer as well. In aerobic cultures, the oxygen mass transfer is still a bottleneck, particularly at industrial scale-up, where the high-cell-density biomass and production of metabo...