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DRhoGEF2 regulates cellular tension and cell pulsations in the Amnioserosa duri...
Azevedo, Dulce; Antunes, Marco; Prag, Soren; Ma, Xiaoyan; Hacker, Udo; Brodland, G. Wayne; Hutson, M. Shane; Solon, Jerome; Jacinto, AntónioCoordination of apical constriction in epithelial sheets is a fundamental process during embryogenesis. Here, we show that DRhoGEF2 is a key regulator of apical pulsation and constriction of amnioserosal cells during Drosophila dorsal closure. Amnioserosal cells mutant for DRhoGEF2 exhibit a consistent decrease in amnioserosa pulsations whereas overexpression of DRhoGEF2 in this tissue leads to an increase in t...
Expression of YAP4 in Saccharomyces cerevisiae under osmotic stress
Nevitt, Tracy; Pereira, Jorge; Azevedo, Dulce; Guerreiro, Paulo; Rodrigues-Pousada, ClaudinaYAP4, a member of the yeast activator protein (YAP) gene family, is induced in response to osmotic shock in the yeast Saccharomyces cerevisiae. The null mutant displays mild and moderate growth sensitivity at 0.4 M and 0.8 M NaCl respectively, a fact that led us to analyse YAP4 mRNA levels in the hog1 (high osmolarity glycerol) mutant. The data obtained show a complete abolition of YAP4 gene expression in this ...
Analysis of a 17.9 kb region from Saccharomyces cerevisiae chromosome VII revea...
Feroli, Fiorella; Carignani, Giovanna; Pavanello, Anna; Guerreiro, Paulo; Azevedo, Dulce; Rodrigues-Pousada, Claudina; Melchioretto, PasqualeWe report the nucleotide sequence of a 17,893 bp DNA segment from the right arm of Saccharomyces cerevisiae chromosome VII. This fragment begins at 482 kb from the centromere. The sequence includes the BRF1 gene, encoding TFIIIB70, the 5' portion of the GCN5 gene, an open reading frame (ORF) previously identified as ORF MGA1, whose translation product shows similarity to heat-shock transcription factors and fiv...
Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals...
Guerreiro, Paulo; Azevedo, Dulce; Barreiros, Tânia; Rodrigues-Pousada, ClaudinaA 9.9 kb DNA fragment from the right arm of chromosome VII of Saccharomyces cerevisiae has been sequenced and analysed. The sequence contains four open reading frames (ORFs) longer than 100 amino acids. One gene, PFK1, has already been cloned and sequenced and the other one is the probable yeast gene coding for the beta-subunit of the succinyl-CoA synthetase. The two remaining ORFs share homology with the deduc...
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