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The first crystal structure of class III superoxide reductase from Treponema pa...
Santos-Silva, Teresa; Trincão, José; Carvalho, Ana Luísa; Bonifácio, Cecília; Auchère, Françoise; Raleiras, Patrícia; Moura, Isabel; Moura, José J. G.J Biol Inorg Chem (2006) 11: 548–558 DOI 10.1007/s00775-006-0104-y ; Superoxide reductase (SOR) is a metalloprotein containing a non-heme iron centre, responsible for the scavenging of superoxide radicals in the cell. The crystal structure of Treponema pallidum (Tp) SOR was determined using soft X-rays and synchrotron radiation. Crystals of the oxidized form were obtained using poly(ethylene glycol) and MgCl2 ...
Kinetics studies of the superoxide-mediated electron transfer reactions between...
Auchère, Françoise; Pauleta, Sofia R.; Tavares, Pedro; Moura, Isabel; Moura, José J. G.J Biol Inorg Chem (2006) 11: 433–444 DOI 10.1007/s00775-006-0090-0 ; In this work we present a kinetic study of the superoxide-mediated electron transfer reactions between rubredoxin-type proteins and members of the three different classes of superoxide reductases (SORs). SORs from the sulfate-reducing bacteria Desulfovibrio vulgaris (Dv) and D. gigas (Dg) were chosen as prototypes of classes I and II, respect...
Superoxide reductase from the syphilis spirochete Treponema pallidum
Moura, Isabel; Santos-Silva, Teresa; Trincão, José; Carvalho, Ana L.; Auchère, Françoise; Moura, José J. G.; Romão, Maria J.Superoxide reductase is a 14 kDa metalloprotein containing a catalytic nonhaem iron centre [Fe(His)4Cys]. It is involved in defence mechanisms against oxygen toxicity, scavenging superoxide radicals from the cell. The oxidized form of Treponema pallidum superoxide reductase was crystallized in the presence of polyethylene glycol and magnesium chloride. Two crystal forms were obtained depending on the oxidizing ...
Overexpression and purification of Treponema pallidum rubredoxin; kinetic evide...
Auchère, Françoise; Sikkink, Robert; Cordas, Cristina; Raleiras, Patrícia; Tavares, Pedro; Moura, Isabel; Moura, José J. G.J Biol Inorg Chem (2004) 9: 839–849 DOI 10.1007/s00775-004-0584-6 ; Superoxide reductases are a class of non-haem iron enzymes which catalyse the monovalent reduction of the superoxide anion O2- into hydrogen peroxide and water. Treponema pallidum (Tp), the syphilis spirochete, expresses the gene for a superoxide reductase called neelaredoxin, having the iron protein rubredoxin as the putative electron donor n...
Formation of a stable cyano-bridged dinuclear iron cluster following oxidation ...
Auchère, Françoise; Raleiras, Patrícia; Benson, Linda; Tavares, Pedro; Moura, José J. G.; Moura, Isabel; Rusnak, FrankInorg. Chem., 2003, 42 (4), pp 938–940 DOI: 10.1021/ic0262886 ; Superoxide reductases catalyze the monovalent reduction of superoxide anion to hydrogen peroxide. Spectroscopic evidence for the formation of a dinuclear cyano-bridged adduct after K(3)Fe(CN)(6) oxidation of the superoxide reductases neelaredoxin from Treponema pallidum and desulfoferrodoxin from Desulfovibrio vulgaris was reported. Oxidation with...
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