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Adventitious root formation in olive (Olea europaea L.) microshoots: anatomical...
Mecedo, E.; Vieira, C.; Carrizo, D.; Porfirio, S.; Hegewald, H.; Arnholdt-Schmitt, B.; Calado, M.L.; Peixe, A.Trials were performed using in vitro-cultured microshoots of the olive (Olea europaea L.) cultivar ‘Galega vulgar’, as initial explants, to identify histological events and modifications in peroxidase and polyphenol oxidase activities during adventitious root formation. Explant bases were submitted to a 10 s quick-dip treatment to promote rooting, using a sterile solution of 14.7 mM indole-3-butyric acid (IBA)....
Formação de raízes adventícias em microestacas de oliveira (Olea europaea L.); ...
Macedo, E.; Vieira, C.; Carrizo, D.; Porfírio, S.; Holger, H.; Arnholdt-Schmitt, B.; Calado, M.L.; Peixe, A.Com o objetivo de identificar tanto fenómenos histológicos como alterações na atividade das peroxidases e polifenoloxidases durante a formação de raízes adventícias, em oliveira realizaram-se ensaios utilizando microestacas da cultivar ‘Galega vulgar’ previamente instalada em cultura in vitro. As bases dos explantes foram submetidas a um tratamento indutor do enraizamento, por imersão rápida (10 segundos) numa ...
Involvement of alternative oxidase (AOX) in adventitious rooting of Olea europa...
Santos Macedo, E.; Sircar, D.; Cardoso, H.G.; Peixe, A; Arnholdt-Schmitt, BAlternative oxidase (AOX) has been proposed as a functional marker candidate in a number of events involving cell differentiation, including rooting efficiency in semi-hardwood shoot cuttings of olive (Olea europaea L.). To ascertain the general importance of AOX in olive rooting, the auxin-induced rooting process was studied in an in vitro system for microshoot propagation. Inhibition of AOX by salicylhydroxa...
Expression studies of AOX during adventitious root induction in Olea europaea L.
Macedo, E.S.; Cardoso, H.G.; Peixe, A.; Arnholdt-Schmitt, B.Recently, identification of four alternative oxidase (AOX) gene sequences from olive (Olea europaea L.) has been reported from our group. Three genes could be classified as members of the sub-family AOX1 and one as belonging to the sub-family of AOX2. AOX has been proposed as functional marker candidate for easy-to-root olive genotypes. Validation of AOX as an appropriate marker candidate needs to highlight a c...
Daucus carota L. – An old model for cell reprogramming gains new importance thr...
Costa, J. H.; Cardoso, H. G.; Campos, M.D.; Zavattieri, A.; Frederico, A. M.; Fernandes de Melo, D.; Arnholdt-Schmitt, B.The paper highlights Daucus carota L. as an ideal model to complement plant stress research on Arabidopsis thaliana L. Recently, alternative oxidase (AOX) is discussed as functional marker candidate for cell reprogramming upon stress. Carrot is the most studied species for cell reprogramming and our current research reveals that it is the only one that has expanded both AOX sub-family genes. We point to recentl...
Isolation of alternative oxidase (AOX) genes of Olea europaea L.
Santos Macedo, E.; Cardoso, H.C.G.; Peixe, A.; Arnholdt-Schmitt, B.Alternative oxidase (AOX) is recently suggested to be a potential candidate as functional marker for efficient cell reprogramming under stress (Arnholdt-Schmitt et al., 2006a). The presented work is part of a Marie Curie Chair project, that was established to investigate the potential role of the multigene AOX to assist breeding on efficient rooting of olive shoot cuttings (Arnholdt-Schmitt et al. 2006b). Plant...
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