Encontrados 8 documentos, a visualizar página 1 de 1

Nanostructured multilayer compartments : towards multifunctionality and ‘‘cell-...

In living organisms, there are phenomena that require the presence of specific biomolecules with distinct function and in variable concentra- tions at a given time, such as the healing and regeneration of tissue and organ lesions. In this work, we propose the use of a compartment- ed drug delivery device for the multiple release of bioactive agents. It consists of nanostructured microcapsules confined within a ...

Self-assembled microcapsules with tunable and sustained release of macromolecul...

Layer-by-layer (LbL) has been presented as a tool capable of constructing tunable and sustained release reservoirs for therapeutic and proliferation/differentiation agents. We report the conception of biocompatible stimuli-responsive microcapsules fabricated using LbL containing BSA as model protein. The capsules were constructed by the sequential adsorption of chitosan and a temperature-responsive elastin-like...

Nanostructured multilayer compartments : towards multifunctionality and ‘‘cell-...

In living organisms, there are phenomena that require the presence of specific biomolecules with distinct function and in variable concentrations at a given time, such as the healing and regeneration of tissue and organ lesions. In this work, we propose the use of a compartmented drug delivery device for the multiple release of bioactive agents. It consists of nanostructured microcapsules confined within a mill...

Layer-by-layer assembly of chitosan and recombinant biopolymers into biomimetic...

In this work, biomimetic smart thin coatings using chitosan and a recombinant elastin-like recombinamer (ELR) containing the cell attachment sequence arginine–glycine–(aspartic acid) (RGD) are fabricated through a layer-by-layer approach. The synthetic polymer is characterized for its molecular mass and composition using mass spectroscopy and peptide sequencing. The adsorption of each polymeric layer is followe...

Temperature-responsive microcapsules prepared by nano-structured multilayers of...

Objectives: Polyelectrolyte vesicles using layer-by-layer (LbL) were recently intro-duced for the encapsulation of therapeutic molecules. This work presents multilay-ered microcapsules of chitosan and a temperature-responsive elastin-like recom-binamer (ELR) as a novel drug delivery system. The release of a pre-loaded model protein was studied at distinct temperatures and number of layers to evaluate the perm...

Exploiting the sequence of naturally occurring elastin : construction, producti...

Genetic engineering was used to produce an elastin-like polymer (ELP) with precise amino acid composition, sequence and length, resulting in the absolute control of MW and stereochemistry. A synthetic monomer DNA sequence encoding for (VPAVG)20, was used to build a library of concatemer genes with precise control on sequence and size. The higher molecular weight polymer with 220 repeats of VPAVG was biologi...

Recombinant elastin‐like polymers : from the design towards application

Construction and bioproduction of a "green" synthetic protein-based polymer exh...