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Antioxidant effect of flavonoids after ascorbate/Fe2+-induced oxidative stress ...
Areias, Filipe Miguel; Rego, A. Cristina; Oliveira, Catarina R.; Seabra, Rosa MariaIn this study, we investigated the structure-activity relationship of four flavonoids, i.e. eriodictyol, luteolin, quercetin, and taxifolin, in cultured retinal cells after ascorbate/Fe2+-induced oxidative stress. The relative order of antioxidant efficacy, determined by the thiobarbituric acid method, was the following: eriodictyol > quercetin > luteolin > taxifolin. Upon preincubation, the flavonoids were als...
Glutamate regulates the viability of retinal cells in culture
Rego, A. Cristina; Santos, Maria Sancha; Areias, Filipe; Proença, Teresa; Oliveira, Catarina R.In this study, we show that glutamate regulates the viability of cultured retinal cells upon transient glucose deprivation. At low concentrations (10-100 [mu]M) glutamate decreased MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] reduction to about 50% of control and decreased intracellular ATP levels (about 4-fold) after transient glucose removal. Under these conditions, the decrease in MTT r...
Distinct Glycolysis Inhibitors Determine Retinal Cell Sensitivity to Glutamate-...
Rego, Ana Cristina; Areias, Filipe; Santos, Maria Sancha; Oliveira, CatarinaIn this study, we analyzed how distinct glycolysis inhibitors influenced the redox status of retinal cells, used as a neuronal model. Three different approaches were used to inhibit glycolysis: the cells were submitted to iodoacetic acid (IAA), an inhibitor of glyceraldehyde 3-phosphate dehydrogenase, to 2-deoxy-glucose (DG) in glucose-free medium, which was used as a substitute of glucose, or in the absence of...
Effect of Glucose Deprivation and Acute Glutamate Exposure in Cultured Retinal ...
Rego, Ana Cristina; Areias, Filipe Miguel; Santos, Maria Sancha; Oliveira, Catarina R.The relationship between bioenergetics and the glutamate system was analyzed in a neuronal model of retinal cells in culture, submitted to glucose deprivation and exposed to glutamate for 2 h, and compared with exposure to glutamate in the presence of glucose. Under glucose deprivation, a reduction (about 1.1-fold) in the energy charge of the cells occurred, probably as a result of a decrement (by about 75%) in...
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