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Quantitative image analysis as a diagnostic tool for identifying structural cha...
Araya-Kroff, P.Tese de Doutoramento em Engenharia Química e Biológica ; In this thesis quantitative image analysis techniques were applied to monitor structural changes of anaerobic sludge under different operating conditions. In a first experiment that lasted 177 days, the phenomenon of aggregation of dispersed sludge was quantitatively monitored through the recognition and quantification of aggregates and free filaments. F...
Quantitative image analysis as a diagnostic tool for identifying structural cha...
Abreu, A. A.; Costa, J. C.; Araya-Kroff, P.; Ferreira, E. C.; Alves, M. M.Due to unspecified operational problems, the specific acetoclastic activity (SAA) of the anaerobic granular sludge present in an industrial UASB reactor was considerably damaged (from 250 to less than 10mL CH4@STP/gVSS.d), significantly reducing the biogas production of that industrial unit. The hydrogenotrophic methanogenic activity exhibited a value of 600mL CH4@STP/gVSS.d, the settling velocity was 31.479.8 ...
Análisis de imagen para la cuantificación de cambios morfológicos en biomasa gr...
Araya-Kroff, P.; Amaral, A. L.; Mota, M.; Ferreira, E. C.; Alves, M. M.Cuatro reactores EGSB (R1-R4) fueron operados a velocidades de carga orgánica de 10 kgCOD/(m³.d), tiempos de retención hidráulicos de 3.6 h, velocidades superficiales de 10 m/h y diferentes concentraciones de nitrato a la entrada, 0, 20, 60 y 100 mgN-NO3-/L en R1, R2, R3 and R4, respectivamente. Después de 20 días de operación continua, la biomasa granular fue caracterizada en términos de: (i) morfología por an...
Recovery of acetoclastic activity in anaerobic granular sludge, monitored by me...
Abreu, A. A.; Costa, J. C.; Araya-Kroff, P.; Ferreira, E. C.; Alves, M. M.The specific acetoclastic activity (SAA) of anaerobic granular sludge was recovered in a lab scale EGSB reactor. The inoculum presented a very low acetoclastic activity (0.81+/-2.48mg COD-CH4/gVSS.day). Ethanol was fed at a COD concentration of 1.5g/L, as sole organic carbon source to promote the biogas production through the hydrogenophilic pathway. During the operation period the specific methanogenic activit...
Development of image analysis techniques as a tool to detect and quantify morph...
Araya-Kroff, P.; Amaral, A. L.; Neves, L.; Ferreira, E. C.; Pons, M. N.; Mota, M.; Alves, M. M.Image analysis techniques were developed and applied to quantify the process of anaerobic granulation in an expanded granular sludge blanket reactor (EGSB) fed with a synthetic substrate based on glucose [60-30% COD chemical oxygen demand)] and volatile fatty acids (40-70% COD) over 376 days. In a first operation period that lasted 177 days, the aggregation of dispersed sludge was quantitatively monitored throu...
Image analysis to quantify morphological changes in granular sludge induced by ...
Araya-Kroff, P.; Amaral, A. L.; Mota, M.; Ferreira, E. C.; Alves, M. M.Four EGSB reactors (R1 to R4) were operated at an organic loading rate of 10 kgCOD/(m³.d), hydraulic retention time of 3.6 h, upflow velocity of 10 m/h and different influent nitrate concentrations (0, 20, 60 and 100 mgN-NO_3-/L for R1, R2, R3 and R4, respectively. After about 20 days of operation, the granular sludge was characterized for: (i) morphology by quantitative image analysis; (ii) specific methanogen...
Monitoring granular stability in an industrial UASB reactor treating brewery wa...
Araya-Kroff, P.; Amaral, A. L.; Ferreira, E. C.; Silva, P. M.; Mota, M.; Alves, M. M.An industrial scale UASB reactor treating a brewery wastewater was monitored during several months, in order to assess the usefulness of a morphological parameter relating the filaments length to the projected area of aggregates, LfA, to anticipate washout problems. It was observed that a selective washout of filamentous bacterial forms could explain the decrease of the acetoclastic and hydrogenophilic activity...
Efeito da diluição na caracterização da biomassa de sistemas de tratamentos de ...
Motta, Maurício da; Amaral, A. L.; Neves, L.; Araya-Kroff, P.; Ferreira, E. C.; Alves, M. M.; Mota, M.; Roche, N.; Vivier, H.; Pons, M. N.O estudo da biomassa de sistemas de tratamento de efluentes por análise de imagem permite avaliar o seu estado de agregação assim como acompanhar as modificações por ela sofrida quando submetida a choques orgânicos ou hidráulicos. Certos processos de tratamento trabalham com uma forte concentração em biomassa, o que obriga a uma diluição prévia. O presente estudo avalia o efeito desta diluição sobre a caracteri...
Image analysis as a tool to recognize anaerobic granulation time
Araya-Kroff, P.; Amaral, A. L.; Neves, L.; Ferreira, E. C.; Alves, M. M.; Mota, M.Image analysis techniques were used to monitor the development of anaerobic granules into an EGSB reactor fed with a synthetic substrate based on glucose (30-40% COD) and volatile fatty acids (70-60% COD) during 400 days. Two types of objects were recognized and quantified: aggregates and free filaments. The aggregates were classified into five ranges based on the equivalent diameter: smaller than 11.3 mm (resi...
Biodegradability and toxicity of styrene in the anaerobic digestion process
Araya-Kroff, P.; Chamy, Rolando; Mota, M.; Alves, M. M.Start-up and operation of an Upflow Anaerobic Sludge Blanket (UASB) reactor fed with an industrial effluent from a polymer synthesis plant containing 6 mg styrene 1ˉ¹ was unstable. In batch assays with 200 mg styrene 1ˉ¹, 74% of styrene was degraded at a rate of 7 ml methane gˉ¹ volatile suspended solids.day, without a lag phase. The toxicity limit (IC50) of styrene was 1.4 mM for the acetoclastic activity, 0.4...
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