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Kinetic behavior of Desulfovibrio gigas aldehyde oxidoreductase encapsulated in...
Moura, José J. G.; Andrade, Susana L. A.; Brondino, Carlos D.; Kamenskaya, Elvira O.; Levashov, Andrey V.Biochemical and Biophysical Research Communications 308 (2003) 73–78 ; We report the kinetic behavior of the enzyme aldehyde oxidoreductase (AOR) from the sulfate reducing bacterium Desulfovibrio gigas (Dg) encapsulated in reverse micelles of sodium bis-(2-ethylhexyl) sulfosuccinate in isooctane using benzaldehyde, octaldehyde, and decylaldehyde as substrates. Dg AOR is a 200-kDa homodimeric protein that catal...
Hydrogen evolution and consumption in AOT–isooctane reverse micelles by Desulfo...
Moura, José J. G.; Andrade, Susana L. A.The enzyme hydrogenase isolated from the sulphate reducing anaerobic bacterium Desulfovibrio gigas was encapsulated in reverse micelles of AOT–water–isooctane. The enzyme ability to consume molecular hydrogen was studied as a function of the micelle size (given by Wo = [H2O]/[organic solvent]). A peak of catalytic activity was obtained for Wo = 18, a micelle size theoretically fitting the heterodimeric hydrogen...
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