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Bacterial cellulose modified using recombinant proteins to improve neuronal and...
Pértile, Renata Aparecida Nedel; Moreira, Susana; Andrade, Fábia K.; Domingues, Lucília; Gama, F. M.A wide variety of biomaterials and bioactive molecules have been applied as scaffolds in neuronal tissue engineering. However, creating devices that enhance the regeneration of nervous system injuries is still a challenge, due the difficulty in providing an appropriate environment for cell growth and differentiation and active stimulation of nerve regeneration. In recent years, bacterial cellulose (BC) has em...
Development of structures based on bacterial cellulose for the production of va...
Andrade, Fábia K.Tese de doutoramento em Engenharia Biomédica ; Cardiovascular disease is the leading cause of mortality in Western countries. For the reconstruction of arteries with large caliber currently available synthetic grafts offer a reasonable solution and proven clinical efficacy. However, for small sized (<6 mm) grafts these materials generally give poor performance, due to anastomotic intimal hyperplasia and surfac...
Improving bacterial cellulose for blood vessel replacement: functionalization w...
Andrade, Fábia K.; Costa, Raquel; Domingues, Lucília; Soares, Raquel; Gama, F. M.Chimeric proteins containing a cellulose-binding module (CBM) and an adhesion peptide (RGD or GRGDY) were produced and used to improve the adhesion of human microvascular endothelial cells (HMEC) to bacterial cellulose (BC). The effect of these proteins on the HMEC–BC interaction was studied. The results obtained demonstrated that recombinant proteins containing adhesion sequences were able to significantly inc...
Surface modification of bacterial cellulose by nitrogen-containing plasma for i...
Pértile, Renata Aparecida Nedel; Andrade, Fábia K.; Alves Junior, Clodomiro; Gama, F. M.Bacterial cellulose (BC) membranes were modified with nitrogen plasma in order to enhance cell affinity. The surface properties of the untreated and plasma modified BC (BCP) were analyzed through contact angle measurements, X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). The effect of the plasma treatment on the adhesion of microvascular (HMEC-1), neuroblast (N1E-115) and fibrobla...
Improving the affinity of fibroblasts for bacterial cellulose using carbohydrat...
Andrade, Fábia K.; Moreira, Susana; Domingues, Lucília; Gama, F. M.The attachment of cells to biomedical materials can be improved by using adhesion sequences, such as Arg-Gly-Asp (RGD), found in several extracellular matrix proteins. In this work, bifunctional recombinant proteins, with a Cellulose-Binding Module (CBM), from the cellulosome of Clostridium thermocellum and cell binding sequences - RGD, GRGDY - were cloned and expressed in E.coli. These RGD-containing cellulose...
Development of a strategy to functionalize a dextrin-based hydrogel for animal ...
Moreira, Susana; Andrade, Fábia K.; Domingues, Lucília; Gama, F. M.Several approaches can be used to functionalize biomaterials, such as hydrogels, for biomedical applications. One of the molecules often used to improve cells adhesion is the peptide Arg-Gly-Asp (RGD). The RGD sequence, present in several proteins from the extra-cellular matrix (ECM), is a ligand for integrin-mediated cell adhesion; this sequence was recognized as a major functional group responsible for cellul...
Production of recombinant carbohydrate-binding modules fused to RGD : functiona...
Andrade, Fábia K.; Moreira, Susana; Domingues, Lucília; Gama, F. M.The attachment of cells to biomedical materials can be improved by using adhesion molecules, present in the extracellular matrix substances, such as fibronectin, vitronectin, or laminin. In many cases, Arg-Gly-Asp (RGD) was found to be the major functional amino acid sequence responsible for cellular adhesion. In the present study, a method for producing chimerics proteins, RGDCBM, with functions similar to fib...
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