Encontrados 11 documentos, a visualizar página 1 de 2
Diabetes induces early transient changes in the content of vesicular transporte...
Baptista, Filipa I.; Gaspar, Joana M.; Cristóvão, Armando; Santos, Paulo F.; Köfalvi, Attila; Ambrósio, António F.Diabetes induces changes in neurotransmitter release in central nervous system, which depend on the type of neurotransmitter and region studied. In this study, we evaluated the effect of diabetes (two and eight weeks duration) on basal and evoked release of [14C]glutamate and [3H]GABA in hippocampal and retinal synaptosomes. We also analyzed the effect of diabetes on the protein content of vesicular glutamate a...
Diabetes changes the levels of ionotropic glutamate receptors in the rat retina
Santiago, Ana R.; Gaspar, Joana M.; Baptista, Filipa I.; Cristóvão, Armando J.; Santos, Paulo F.; Kamphuis, Willem; Ambrósio, António F.Purpose: Diabetic retinopathy (DR) is a leading cause of vision loss and blindness among adults between the age 20 to 74. Changes in ionotropic glutamate receptor subunit composition can affect retinal glutamatergic neurotransmission and, therefore, contribute to visual impairment. The purpose of this study was to investigate whether diabetes leads to changes in ionotropic glutamate receptor subunit expression ...
Diabetes changes ionotropic glutamate receptor subunit expression level in the ...
Santiago, Ana R.; Hughes, John M.; Kamphuis, Willem; Schlingemann, Reinier O.; Ambrósio, António F.Early diabetic retinopathy is characterized by changes in subtle visual functions such as contrast sensitivity and dark adaptation. The outcome of several studies suggests that glutamate is involved in retinal neurodegeneration during diabetes. We hypothesized that the protein levels of ionotropic glutamate receptor subunits are altered in the retina during diabetes. Therefore, we investigated whether human dia...
High glucose and oxidative/nitrosative stress conditions induce apoptosis in re...
Leal, Ermelindo C.; Aveleira, Célia A.; Castilho, Áurea F.; Serra, Andreia M.; Baptista, Filipa I.; Hosoya, Ken-Ichi; Forrester, John V.http://www.sciencedirect.com/science/article/B6WFD-4V94X19-2/2/49680e499c2d31bd74eb7ad7fc99a8c2
Early calpain-mediated proteolysis following AMPA receptor activation compromis...
Araújo, Inês M.; Verdasca, Maria J.; Leal, Ermelindo C.; Bahr, Ben A.; Ambrósio, António F.; Carvalho, Arsélio P.; Carvalho, Caetana M.http://dx.doi.org/10.1111/j.1471-4159.2004.03043.x
Mechanisms of Action of Carbamazepine and Its Derivatives, Oxcarbazepine, BIA 2...
Ambrósio, António F.; Soares-da-Silva, Patrício; Carvalho, Caetana M.; Carvalho, Arsélio P.Carbamazepine (CBZ) has been extensively used in the treatment of epilepsy, as well as in the treatment of neuropathic pain and affective disorders. However, the mechanisms of action of this drug are not completely elucidated and are still a matter of debate. Since CBZ is not very effective in some epileptic patients and may cause several adverse effects, several antiepileptic drugs have been developed by struc...
Inhibition of glutamate release by BIA 2-093 and BIA 2-024, two novel derivativ...
Ambrósio, António F.; Silva, Ana P.; Malva, João O.; Soares-da-Silva, Patricio; Carvalho, Arsélio P.; Carvalho, Caetana M.We investigated the mechanism(s) of action of two new putative antiepileptic drugs (AEDs), (S)-(-)-10-acetoxy-10,11-dihydro-5H-dibenz[b,f]azepine-5-carboxamide (BIA 2-093) and 10,11-dihydro-10-hydroxyimino-5H-dibenz[b,f]azepine-5-carboxamide (BIA 2-024), by comparing their effects on the release of endogenous glutamate in hippocampal synaptosomes, with those of carbamazepine (CBZ) and oxcarbazepine (OXC). The A...
Role of kainate receptor activation and desensitization on the [Ca2+]i changes ...
Silva, Ana P.; Malva, João O.; Ambrósio, António F.; Salgado, António J.; Carvalho, Arsélio P.; Carvalho, Caetana M.We investigated the role of kainate (KA) receptor activation and desensitization in inducing the increase in the intracellular free Ca2+ concentration ([Ca2+]i) in individual cultured rat hippocampal neurons. The rat hippocampal neurons in the cultures were shown to express kainate receptor subunits, KA2 and GluR6/7, either by immunocytochemistry or by immunoblot analysis. The effect of LY303070, an alpha-amino...
Neurotoxic/neuroprotective profile of carbamazepine, oxcarbazepine and two new ...
Ambrósio, António F.; Silva, Ana P.; Araújo, Inês; Malva, João O.; Soares-da-Silva, Patrício; Carvalho, Arsélio P.; Carvalho, Caetana M.We investigated and compared the toxicity profile, as well as possible neuroprotective effects, of some antiepileptic drugs in cultured rat hippocampal neurons. We used two novel carbamazepine derivatives, (S)-(-)-10-acetoxy-10,11-dihydro-5H-dibenz[b,f]azepine-5-carboxamide (BIA 2-093) and 10,11-dihydro-10-hydroxyimino-5H-dibenz[b,f]azepine-5-carboxamide (BIA 2-024), and compared their effects with the establis...
Carbamazepine inhibits L-type Ca2+ channels in cultured rat hippocampal neurons...
Ambrósio, António F.; Silva, Ana P.; Malva, João O.; Soares-da-Silva, Patrício; Carvalho, Arsélio P.; Carvalho, Caetana M.In order to better understand the mechanism(s) of action of carbamazepine (CBZ), we studied its effects on the increase in [Ca2+]i and [Na+]i stimulated by glutamate ionotropic receptor agonists, in cultured rat hippocampal neurons, as followed by indo-1 or SBFI fluorescence, respectively. CBZ inhibited the increase in [Ca2+]i stimulated either by glutamate, kainate, [alpha]-amino-3-hydroxy-5-methyl-isoxazole-4...
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