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Competition between lithium and magnesium ions for the G-protein transducin in ...
Srinivasan, Chandra; Toon, Jason; Amari, Louis; Abukhdeir, Abde M.; Hamm, Heidi; Geraldes, Carlos F. G. C.; Ho, Yee-Kin; Freitas, Duarte Mota deLi+ is the most effective drug used to treat bipolar disorder; however, its exact mechanism of action has yet to be elucidated. One hypothesis is that Li+ competes with Mg2+ for the Mg2+ binding sites on guanine-nucleotide binding proteins (G-proteins). Using 7Li T1 relaxation measurements and fluorescence spectroscopy with the Mg2+ fluorophore furaptra, we detected Li+/Mg2+ competition in three preparations: t...
Comparison of Fluorescence, 31P NMR, and 7Li NMR Spectroscopic Methods for Inve...
Amari, Louis; Layden, Brian; Rong, Qinfen; Geraldes, Carlos F. G. C.; Freitas, Duarte Mota deThe biochemical action of lithium in the treatment of manic-depressive illness is still unknown. One hypothesis is that Li+ competes for Mg2+-binding sites in biomolecules. We report here our studies on metal ion competition by three distinct methods: fluorescence, 31P NMR, and 7Li NMR spectroscopy, using ATP as a model ligand. By fluorescence spectroscopy, we used the dye, furaptra, by measuring the increases ...
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