Encontrados 11 documentos, a visualizar página 1 de 2
Mineral stress affects the cell wall composition og grapevine (Vitis vinifera L...
Fernandes, João C.; Garcia-Angulo, Penélope; Goulão, Luis F.; Acebes, José L.; Amâncio, SaraVersão provisória aceite p. publicação na Plant Science (ISSN 0168-9452)em 19-01-2013 ; Grapevine (Vitis vinifera L.) is one of the most economically important fruit crops in the world. Deficit in nitrogen, phosphorus and sulfur nutrition impairs essential metabolic pathways. The influence of mineral stress in the composition of the plant cell wall (CW) has received residual attention. Using grapevine callus a...
Microarray-based uncovering reference genes for quantitative real time PCR in g...
Coito, João L.; Rocheta, Margarida; Carvalho, Luísa; Amâncio, SaraResearch Article ; Background: Quantitative real time polymerase chain reaction is becoming the primary tool for detecting mRNA and transcription data analysis as it shows to have advantages over other more commonly used techniques. Nevertheless, it also presents a few shortcomings, with the most import being the need for data normalisation, usually with a reference gene. Therefore the choice of the reference ...
Tackling the cell wall of the grape berry
Goulão, L.F.; Fernandes, J.C.; Lopes, P.; Amâncio, SaraAbstract: The cell wall (CW) is the dynamic border of plant cells. In grape berries, the CW decisively accounts for the difference between the pulp and skin cells, with direct consequences on the grape characteristics, wine quality and wine-making methods. The softening of mature berries results from the depolymerisation and solubilisation of CW polymers. Modifications of grape pulp and skin CW provide the flex...
Comparative transcriptomic profiling of Vitis vinifera under high light using a...
Carvalho, Luísa C.; Vilela, Belmiro J.; Mullineaux, Phil M.; Amâncio, SaraResearch Article ; Understanding abiotic stress responses is one of the most important issues in plant research nowadays. Abiotic stress, including excess light, can promote the onset of oxidative stress through the accumulation of reactive oxygen species. Oxidative stress also arises when in vitro propagated plants are exposed to high light upon transfer to ex vitro. To determine whether the underlying pathwa...
Comparative transcriptomic profiling of Vitis vinifera under high light using a...
Carvalho, Luísa C.; Vilela, Belmiro J.; Mullineaux, Phil M.; Amâncio, SaraABSTRACT Understanding abiotic stress responses is one of the most important issues in plant research nowadays. Abiotic stress, including excess light, can promote the onset of oxidative stress through the accumulation of reactive oxygen species. Oxidative stress also arises when in vitro propagated plants are exposed to high light upon transfer to ex vitro. To determine whether the underlying pathways activate...
Efeito da casta no enraizamento de estacas semilenhosas de videira
Brazão, João; Gonçalves, Elsa; Eiras-Dias, José; Amâncio, SaraFrom the massal and clonal selection of grapevine developed in Portugal for the last 30 years, clones of several varieties with high genetic potential were obtained, which are currently used for multiplication to produce base and certified materials. In order to allow the rapid multiplication of selected clones of grapevine, this work evaluate the effect of the variety (Arinto, Malvasia Fina, Aragonez, and Trin...
Sugarcane (Saccharum sp. Hybrid) propagated in headspace renovating systems sho...
Aragón, Carlos; Carvalho, Luísa A.; González, Justo; Escalona, Maritza; Amâncio, SaraPrevious results have shown that sugarcane plantlets micropropagated in Temporary Immersion Bioreactors (TIB) demonstrated a better morphology and physiological behaviour when compared to plantlets propagated in Gelled Medium (GM). The present work focuses on the onset of oxidative stress symptoms at transfer to ex vitro and during acclimatization. The specific ROS being produced were identified and tissue-loca...
Integration of stress produced reactive oxygen species in the stomatal regulati...
Vilela, B.J.; Carvalho, L.C.; Amâncio, SaraArticle Addendum ; Reactive oxygen species (ROS) have been repeatedly implicated as cellular second messengers important in the modulation of almost every ontogenic phase of plant development, from seedling to cell death. In all of these processes, ROS production and detoxification are highly regulated and its levels are kept under tight control, in order to achieve the desired effect at the cellular or plant ...
Quantification of endogenous levels of IAA, IAAsp and IBA in micro-propagated s...
Gonçalves, José Carlos; Diogo, Graça; Coelho, Maria Teresa; Vidal, Nieves; Amâncio, SaraEndogenous levels of indole-3-acetic acid (IAA), indole-3-acetylaspartic acid (IAAsp) and indole-3-butyric acid (IBA) were measured during the first 8 d of in vitro rooting of rootstock from the chestnut ‘M3’ hybrid by high performance liquid chromatography (HPLC). Rooting was induced either by dipping the basal ends of the shoots into a 4.92-mM IBA solution for 1 min or by sub-culturing the shoots on solid roo...
Solanum lycopersicon Mill. and Nicotiana benthamiana L. under high light show d...
Carvalho, Luísa C.; Santos, Sónia; Vilela, B.J.; Amâncio, SaraTwo experimentally important species, Solanum lycopersicon Mill. and Nicotiana benthamiana L., were propagated in vitro under low light (50 mmolm 2 s 1) and transferred to HL (200 mmolm 2 s 1) under a protocol previously developed for grapevine and chestnut. Compared with photooxidative stress parameters already tested in those species, imaging of hydrogen peroxide and superoxide revealed an accumulation on d2–...
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