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Evolutionary history and population genetics of a cyprinid fish (Iberochondrost...

Abstract The use of molecular techniques has shown that hybridization and introgression have significant impacts in evolution, by means of transfer of genetic variation and formation of hybrid species. In this paper we use mitochondrial and nuclear sequence data to investigate the evolutionary history, levels of genetic diversity and population differentiation of a rare and endangered fish species. Our results ...

Diagnóstico imunológico de doenças associadas a vectores existentes em Portugal

Clinical presentaion and laboratory findings for the first autochthonous cases ...

Lampreys of the Iberian Peninsula: distribution, population status and conserva...

The 3 lamprey species, sea lamprey Petromyzon marinus L., European river lamprey Lampetra fluviatilis L. and European brook lamprey L. planeri Bloch, that inhabit the Iberian Peninsula are of conservation concern. They are considered either Vulnerable, Critically Endangered, and even Extinct in different regions of this area mainly due to habitat loss and population fragmentation. Although several other factors...

Infecção por vírus West Nile [Flavivírus] em Portugal. Considerações acerca de ...

O vírus West Nile [WN] é um flavivírus transmitido por mosquitos e agente etiológico de febre e de doença neuroinvasiva. O vírus WN mantém-se na natureza em ciclos enzoóticos que envolvem mosquitos ornitofílicos, como vectores primários, e algumas espécies de aves como reservatório primário. A sua presença em Portugal é conhecida, surgindo esporadicamente alguns casos de infecção em equinos e humanos. Em 2010 f...

Diagnostic Assays for Crimean-Congo hemorrhagic fever

Crimean-Congo hemorrhagic fever (CCHF) is a highly contagious viral tick-borne disease with case-fatality rates as high as 50%. We describe a collaborative evaluation of the characteristics, performance, and on-site applicability of serologic and molecular assays for diagnosis of CCHF. We evaluated ELISA, immunofluorescence, quantitative reverse transcription PCR, and low-density macroarray assays for detection...

Boletim Agrometeorológico ano 2002 para a sub-região da Nhecolândia, Pantanal, ...

Este boletim tem o intuito de difundir as informações agroclimáticas coletadas no Pantanal da Nhecolândia, visando subsidiar a pesquisa agropecuária da região, além de atender todos àqueles que necessitam de informações dessa natureza. ; 2005 ; Formato Eletrônico

Boletim agrometeorológico ano 2001 para a sub-região da Nhecolândia, Pantanal, ...

Fontes dos dados meteorológicos; Dados meteorológicos e normais climatológicas; Apresentação dos dados meteorológicos; Comparação das normais climatológicos na sub-região da Nhecolândia, Pantanal, MS, entre o período de 1977 a 2000 e 2001; Análise dos dados meteorológicos. ; 2003

Immunocytochemical and biochemical detection of alpha-L-fucosidase in Trypanoso...

The aim of the present study was to demonstrate the presence of alpha-L-fucosidase in Trypanosoma cruzi. Immunocytochemical and biochemical techniques were used to localize and characterize a membrane-associated, neutral-pH-optimum, alpha-L-fucosidase from the parasite. Light and electron microscopy localized the alpha-L-fucosidase specifically on the surface of the parasite and on membranes in the posterior re...

RNA and DNA aptamers as potential tools to prevent cell adhesion in disease

Recent research has shown that receptor-ligand interactions between surfaces of communicating cells are necessary prerequisites for cell proliferation, cell differentiation and immune defense. Cell-adhesion events have also been proposed for pathological conditions such as cancer growth, metastasis, and host-cell invasion by parasites such as Trypanosoma cruzi. RNA and DNA aptamers (aptus = Latin, fit) that hav...