Encontrados 15 documentos, a visualizar página 1 de 2
Responsive and In situ-forming chitosan scaffolds for bone tissue engineering a...
Martins, Ana M.; Alves, C. M.; Kasper, F. Kurtis; Mikos, Antonios G.; Reis, R. L.The use of bioabsorbable polymeric scaffolds is being investigated for use in bone tissue engineering applications, as their properties can be tailored to allow them to degrade and integrate at optimal rates as bone remodelling is completed. The main goal of this review is to highlight the “intelligent” properties exhibited by chitosan scaffolds and their use in the bone tissue engineering field. To complement ...
Combination of enzymes and flow perfusion conditions improves osteogenic differ...
Martins, Ana M.; Saraf, A.; Sousa, R. A.; Alves, C. M.; Mikos, Antonios G.; Kasper, F. Kurtis; Reis, R. L.Previous studies have shown that a-amylase and lipase are capable of enhancing the degradation of fiber meshes blends of starch and poly(e-caprolactone) (SPCL) under dynamic conditions, and consequently to promote the proliferation and osteogenic differentiation of bone marrow stromal cells (MSCs). This study investigated the effect of flow perfusion bioreactor culture in combination with enzymes on the osteoge...
Immobilization of fibronectin in chitosan substrates improves cell adhesion and...
Custódio, Catarina A.; Alves, C. M.; Reis, R. L.; Mano, J. F.Covalent grafting of biomolecules is a strategy to improve the biocompatibility and bioactivity of materials. However, it is critical to maintain the biological activity of the biomolecule upon its attachment to the surface. In the present study we compared the biological properties of chitosan, in which the surface was enriched with fibronectin (Fn), using two methodologies: chemical immobilization, using a wa...
Situação epidemiológica da brucelose bovina no Estado de Minas Gerais
Gonçalves,V.S.P.; Delphino,M.K.V.C.; Dias,R.A.; Ferreira,F.; Amaku,M.; Ferreira Neto,J.S.; Porto,T.B.; Alves,C.M.; Figueiredo,V.C.F.; Lôbo,J.R.Realizou-se um estudo para caracterizar a situação epidemiológica da brucelose bovina no Estado de Minas Gerais. O Estado foi estratificado em sete circuitos produtores. Em cada circuito foram amostradas aleatoriamente cerca de 300 propriedades e, dentro dessas, foi escolhido, de forma aleatória, um número pré-estabelecido de animais, dos quais foi obtida uma amostra de sangue. No total, foram amostrados 20.643...
Toward osteogenic differentiation of marrow stromal cells and in vitro producti...
Martins, Ana M.; Alves, C. M.; Reis, R. L.; Mikos, Antonios G.; Kasper, F. KurtisUncorrected proof ; Tissue engineering has emerged as a new interdisciplinary field for the repair of various tissues, restoring their functions by using scaffolds, cells, and/or bioactive factors. A temporary scaffold acts as an extracellular matrix analog to culture cells and guide the development of new tissue. In this chapter, we discuss the preparation of naturally derived scaffolds of polysaccharide orig...
Effects of protein incorporation on calcium phosphate coating
Leonor, I. B.; Alves, C. M.; Azevedo, Helena S.; Reis, R. L.The incorporation of proteins into calcium phosphate (Ca–P) coatings is expected to alter their properties. The aim of this work is, therefore, to study the effect of protein concentration on the formation of Ca–P film. A biodegradable blend of corn starch/ethylene vinyl alcohol (SEVA-C) was used as substrate and bioactive glass (45S5 Bioglass®) was used as a nucleating agent. Bovine serum albumin (BSA) and α-a...
Surface-modified 3D starch-based scaffold for improved endothelialization for b...
Santos, M. I.; Pashkuleva, I.; Alves, C. M.; Gomes, Manuela E.; Fuchs, Sabine; Unger, Ronald E.; Reis, R. L.; Kirkpatrick, C. JamesProviding adequate vascularization is one of the main hurdles to the widespread clinical application of bone tissue engineering approaches. Due to their unique role in blood vessel formation, endothelial cells (EC) play a key role in the establishment of successful vascularization strategies. However, currently available polymeric materials do not generally support EC growth without coating with adhesive protei...
Plasma surface modification of poly(D,L-lactic acid) as a tool to enhance prote...
Alves, C. M.; Yang, Y.; Marton, D.; Carnes, D. L.; Ong, J. L.; Sylvia, V. L.; Dean, D. D.; Reis, R. L.; Agrawal, C. M.We have studied the influence of oxygen radio frequency glow discharge (RfGD) on the surface and bulk properties of poly(D,L-lactic acid) (PDLLA) and the effect of this surface modification on both protein adsorption and bone cell behavior. PDLLA films were characterized before and after plasma surface modification by water contact angle, surface energy, and adhesion tension of water as well as by scanning elec...
Modulating bone cells response onto starch-based biomaterials by surface plasma...
Alves, C. M.; Yang, Y.; Carnes, D. L.; Ong, J. L.; Sylvia, V. L.; Dean, D. D.; Agrawal, C. M.; Reis, R. L.The effect of oxygen-based radio frequency glow discharge (rfGD) on the surface of different starch-based biomaterials (SBB) and the influence of proteins adsorption on modulating bone–cells behavior was studied. Bovine serum albumin, fibronectin and vitronectin were used in single and complex protein systems. RfGD-treated surfaces showed to increase in hydrophilicity and surface energy when compared to non-mod...
Morphometric analysis of the thymus of puppies infected with the Snyder Hill St...
Alves,C.M.; Vasconcelos,A.C.; Martins,A.S.; del Puerto,H.L.; Santos,F.G.A.; Nunes,J.E.S.; Campos,P.P.; Moro,L.The thymic morphometry analysis was used for determining apoptosis and atrophy of the thymus of eight puppies inoculated with canine distemper virus (CDV). Three healthy dogs were used as negative controls. Sections, 5µm thick, were stained by HE and Shorr, and the latter were evaluated by morphometry. CDV nucleoprotein was detected by immunohistochemistry. Morphometric results confirmed lymphoid hypotrophy in ...
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