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Função Gonadal na Síndrome de Turner
Alves, Márcia; Serviço de Endocrinologia, Diabetes e Metabolismo. Centro Hospitalar e Universitário de Coimbra, EPE. Coimbra. Portugal.Introdução: A síndrome de Turner caracteriza-se pela ausência, parcial ou total, de um cromossoma X no sexo feminino, sendo uma das cromossomopatias mais frequentes. O diagnóstico é realizado através do cariótipo e as suas manifestações incluem o hipogonadismo primário, antes ou após a puberdade (disgenesia gonadal). O grau de disfunção e a extensão dos defeitos gonadais são variáveis.Objectivos: Pretendeu-se a...
Anterior positioning of sex chromosomes on the head of human sperm sorted using...
Alçada-Morais, Sofia; Sousa, Ana Paula; Paiva, Artur; Almeida-Santos, Teresa; Ramalho-Santos, J.The human ejaculate contains subpopulations of sperm with distinct properties. Human X- and Y-bearing sperm were separated with fluorescence activated cell sorting. To avoid the use of UV light the quantitative DNA dyes DRAQ5® and Dyecycle™ Vybrant® Violet were used. Sorting efficiency was similar for both dyes, but lower than what is usually obtained with the classical method involving Hoechst 33342 and UV lig...
Not All Sperm Are Equal: Functional Mitochondria Characterize a Subpopulation o...
Sousa, Ana Paula; Amaral, Alexandra; Baptista, Marta; Tavares, Renata; Caballero Campo, Pedro; Caballero Peregrín, Pedro; Freitas, AlbertinaHuman sperm samples are very heterogeneous and include a low amount of truly functional gametes. Distinct strategies have been developed to characterize and isolate this specific subpopulation. In this study we have used fluorescence microscopy and fluorescence-activated cell sorting to determine if mitochondrial function, as assessed using mitochondrialsensitive probes, could be employed as a criterion to obta...
The indirect effect of contextual factors on the emotional distress of infertil...
Moura-Ramos, Mariana; Gameiro, Sofia; Canavarro, Cristina; Soares, Isabel; Almeida-Santos, TeresaFew studies were dedicated to study the role of contextual factors, such as the socioeconomic status and urban or rural residence in emotional distress of infertile couples. This study aimed to explore the impact of contextual factors on emotional distress, either directly or by affecting the importance of parenthood in one's life, which in turn affects emotional distress. In this cross-sectional study, 70 coup...
Dual use of Diff-Quik-like stains for the simultaneous evaluation of human sper...
Sousa, Ana Paula M.; Tavares, Renata S.; Velez de la Calle, Juan Felipe; Figueiredo, Helena; Almeida, Vasco; Almeida-Santos, TeresaBACKGROUND: Sperm chromatin status and nuclear DNA damage can be detected using well-established assays. However, most techniques are time-consuming and/or involve elaborate protocols and equipment. We have recently developed a simple and fast method to monitor sperm chromatin status in field conditions using the Diff-Quik assay which is employed in fertility clinics to assess sperm morphology with standard bri...
Characterization of human sperm populations using conventional parameters, surf...
Varum, Sandra; Bento, Carla; Sousa, Ana Paula M.; Gomes-Santos, Carina S. S.; Henriques, Paula; Almeida-Santos, Teresa; Teodósio, Cristina; Paiva, ArturTo directly compare distinct assays proposed to monitor human sperm quality and possibly preselect sperm populations for assisted reproductive technology (ART).Design ; http://www.sciencedirect.com/science/article/B6T6K-4MD46F2-4/1/881ded71ecdcac2b19dc8841945d9b66
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