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Evaluation of human sperm chromatin status after selection using a modified Dif...
Tavares, R. S.; Silva, A. F.; Lourenço, B.; Almeida-Santos, T.; Sousa, A. P.; Ramalho-Santos, J.Sperm chromatin/DNA damage can be measured by a variety of assays. However, it has been reported that these tests may lose prognostic value in Assisted Reproductive Technology (ART) cycles when assessed in post-prepared samples, possibly due to the normalizing effect promoted by sperm preparation procedures. We have recently implemented a modified version of the Diff-Quik staining assay that allows for the eval...
Dual use of Diff-Quik-like stains for the simultaneous evaluation of human sper...
Sousa, AP; Tavares, RS; Velez de la Calle, JF; Figueiredo, H; Almeida, V; Almeida-Santos, T; Ramalho-Santos, JBACKGROUND: Sperm chromatin status and nuclear DNA damage can be detected using well-established assays. However, most techniques are time-consuming and/or involve elaborate protocols and equipment. We have recently developed a simple and fast method to monitor sperm chromatin status in field conditions using the Diff-Quik assay which is employed in fertility clinics to assess sperm morphology with standard bri...
Cytogenetic analysis of spontaneously activated noninseminated oocytes and part...
Almeida-Santos, T; Dias, C; Henriques, P; Brito, R; Barbosa, A; Regateiro, FJ; Almeida-Santos, APURPOSE:Spontaneous parthenogenetically activated noninseminated oocytes and failed fertilized oocytes after ART activated by puromycin were studied to assess cleavage ability and the cytogenetic constitution of the resulting embryos. METHODS: Failed fertilized oocytes were exposed to puromycin, and whenever activation occurred, they were further cultured until arrest of development. FISH was used to assess the...
Analysis of human spermatozoa by fluorescence in situ hybridization with preser...
Almeida-Santos, T; Dias, C; Brito, R; Henriques, P; Almeida-Santos, APURPOSE: Development of a hybridization technique for spermatozoa allowing the preservation of the head morphology. METHODS: FISH analysis of fixed semen samples from oligoasthenoteratozoospermia (OAT) patients with a normal somatic karyotype attending the Cytogenetics and the IVF Laboratories of a University hospital for semen analysis. In situ hybridization with centromeric probes for chromosomes X, Y, and 1...
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