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Cytogenetic analysis of spontaneously activated noninseminated oocytes and part...

Almeida-Santos, T; Dias, C; Henriques, P; Brito, R; Barbosa, A; Regateiro, FJ; Almeida-Santos, A

PURPOSE:Spontaneous parthenogenetically activated noninseminated oocytes and failed fertilized oocytes after ART activated by puromycin were studied to assess cleavage ability and the cytogenetic constitution of the resulting embryos. METHODS: Failed fertilized oocytes were exposed to puromycin, and whenever activation occurred, they were further cultured until arrest of development. FISH was used to assess the...


Analysis of human spermatozoa by fluorescence in situ hybridization with preser...

Almeida-Santos, T; Dias, C; Brito, R; Henriques, P; Almeida-Santos, A

PURPOSE: Development of a hybridization technique for spermatozoa allowing the preservation of the head morphology. METHODS: FISH analysis of fixed semen samples from oligoasthenoteratozoospermia (OAT) patients with a normal somatic karyotype attending the Cytogenetics and the IVF Laboratories of a University hospital for semen analysis. In situ hybridization with centromeric probes for chromosomes X, Y, and 1...



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Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia