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Probing the surface chemistry of different oxidized MWCNT for the improved elec...
Silveira, Célia M.; Pimpão, Marta; Pedroso, Humberto A.; Rodrigues, Patrícia R. S.; Moura, José J. G.; Pereira, Manuel F. R.; Almeida, M. Gabriela"This work reports the evaluation of a set of multi-walled carbon nanotubes (MWCNT) presenting different surface chemistries, as interfaces for the direct electrochemistry of the multihemic nitrite reductase (ccNiR) from Desulfovibrio desulfuricans ATCC27774 (Dd). The carbon nanotubes dispersions were prepared in aqueous media and deposited on pyrolytic graphite (PG) macroelectrodes, following a layer-by-layer ...
Small electron transfer proteins as mediators in enzymatic electrochemical bios...
Silveira, Célia M.; Almeida, M. Gabriela"Electrochemical mediators transfer redox equivalents between the active sites of enzymes and electrodes and, in this way, trigger bioelectrocatalytic redox processes. This has been very useful in the development of the so-called second generation biosensors, where they are able to transduce the catalytic event into an electrical signal. Among other pre-requisites, redox mediators must be readily oxidized/reduc...
Nitrite biosensing via selective enzymes—a long but promising route
Almeida, M. Gabriela; Serra, Alexandra; Silveira, Celia M.; Moura, José J. G.Sensors 2010, 10, 11530-11555 ; The last decades have witnessed a steady increase of the social and political awareness for the need of monitoring and controlling environmental and industrial processes. In the case of nitrite ion, due to its potential toxicity for human health, the European Union has recently implemented a number of rules to restrict its level in drinking waters and food products. Although sev...
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