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Surface hydrolysis of polyamide with a new polyamidase from Beauveria brongniartii
Almansa, Eva; Heumann, Sonja; Eberl, A.; Kaufmann, F.; Paulo, Artur Cavaco; Gübitz, Georg M.Twelve fungi were screened for the potential of their extracellular enzymes to increase the hydrophilicity of polyamide (PA) materials. The most pronounced increase in hydrophilicity was found for enzymes from Beauveria brongniartii and B. bassiana. The 55 kDa polyamidase from B. brongniartii was purified using ultrafiltration, anion exchange chromatography and size exclusion chromatography. This polyamidase wa...
New model substrates for enzymes hydrolysing polyethyleneterephthalate and poly...
Heumann, Sonja; Eberel, Anita; Pobeheim, Herbert; Liebminger, Stefan; Fischer-Colbrie, Gudrun; Almansa, Eva; Paulo, Artur Cavaco; Gübitz, Georg M.Recently the potential of enzymes for surface hydrophilisation and/or functionalisation of polyethyleneterephthalate (PET) and polyamide (PA) has been discovered. However, there was no correlation between enzyme class/activity (e.g. esterase, lipase, cutinase) and surface hydrolysis of these polymers and consequently no simple assay to estimate this capability. Enzymes active on the model substrates bis (benzoy...
Influence of structure on de degradation with lacase mediator systems
Almansa, Eva; Andreas, Kandelbauer; Pereira, Luciana; Paulo, Artur CavacoA new laccase was purified from Trametes hirsuta IMA2002. The laccase had a molecular mass of 62 kDa and an isoelectric point of pH 7. It had an optimum pH of 3.0 and an optimum temperature of 558C. The laccase was quite stable at 308C and pH 4.0 with a half-life of more than 100 hours. On ABTS, yringaldazide, and DMP the laccase showed KM and Kcat values of 75, 12 and 37 mM and 64, 83 and 54 s_1, respectively....
New enzymes with potential for PET surface modification
Fischer-Colbrie, Gudrun; Heumann, Sonja; Liebminger, Stefan; Almansa, Eva; Paulo, Artur CavacoThis work describes newly isolated organisms and their potential to modify the surface of polyethylene terephthalate (PET). Out of the different screening processes, four bacterial and five fungal strains were isolated. A PET model substrate was synthesized (bis (benzoyloxyethyl) terephthalate) and used in the screening process, mimicking the polymer in its crucial properties and having the advantage of defined...
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