Encontrados 23 documentos, a visualizar página 1 de 3
Ln[DO3A-N-α-(pyrenebutanamido)propionate] complexes: optimized relaxivity and N...
Ferreira, M. F.; Pereira, G.; Martins, A. F.; Martins, C. I. O.; Prata, M. I. M.; Petoud, S.; Toth, E.; Ferreira, P. M. T.; Martins, J. A.We have proposed recently that the DO3A-N-α-(amino)propionate chelator and its amide conjugates are leads to targeted, high relaxivity, safe contrast agents for magnetic resonance imaging. In this work we illustrate further the expeditious nature and robustness of the synthetic methodologies developed by preparing the DO3A-N-(α-pyrenebutanamido)propionate chelator. Its Gd3+ chelate retains the optimized water e...
Poly(ethylene glycol)-block-poly(4-vinyl pyridine) as a versatile block copolym...
Rocha, Nuno; Mendes, Joana; Durães, Luisa; Maleki, Hajar; Portugal, Antonio; Geraldes, C. F. G. C.; Serra, Arménio; Coelho, JorgeThis work reports an efficient method for the preparation of aqueous dispersions of superparamagnetic iron oxide nanoparticles (SPIONs), involving the use of an amphiphilic block copolymer, poly(ethylene glycol)- block-poly(4-vinyl pyridine) (mPEG-b-P4VP). The iron oxide nanoparticles are easily and efficiently dispersed due to the strong direct interaction of the hydrophobic P4VP segments, through complexation...
New dextrin nanomagnetogels as contrast agents for magnetic resonance imaging
Gonçalves, C.; Lalatonne, Y.; Melro, L.; Badino, G.; Ferreira, M. F. M.; David, L.; Geraldes, C. F. G. C.; Motte, L.; Martins, J. A.; Gama, F. M.This study aims at the production and characterization of a “nanomagnetogel” consisting of superparamagnetic iron oxide nanoparticles (g-Fe2O3) stabilized within a hydrophobized-dextrin nanogel. The nanomagnetogel obtained was extensively characterized with respect to physico-chemical (transmission electron microscopy, cryo-scanning electron microscopy, dynamic light scattering, small angle X-ray scattering), m...
New dextrin nanomagnetogels as contrast agents for magnetic resonance imaging
Gonçalves, Catarina; Lalatone, Y.; Melro, Liliana Sofia da Silva Ferreira Pinto; Badino, G.; Ferreira, M. F. M.; David, L.; Geraldes, C. F. G. C.This study aims at the production and characterization of a “nanomagnetogel” consisting of superparamagnetic iron oxide nanoparticles (γ-Fe2O3) stabilized within a hydrophobized-dextrin nanogel. The nanomagnetogel obtained was extensively characterized with respect to physico-chemical (transmission electron microscopy, cryo-scanning electron microscopy, dynamic light scattering, small angle X-ray scattering), m...
Sources of hepatic triglyceride accumulation during high-fat feeding in the hea...
Delgado, T. C.; Pinheiro, D.; Caldeira, M.; Castro, M. M. C. A.; Geraldes, C. F. G. C.; López-Larrubia, P.; Cerdán, S.; Jones, J. G.Hepatic triglyceride (HTG) accumulation from peripheral dietary sources and from endogenous de novo lipogenesis (DNL) was quantified in adult Sprague-Dawley rats by combining in vivo localized 1H MRS measurement of total hepatic lipid with a novel ex vivo 2H NMR analysis of HTG 2H enrichment from 2H-enriched body water. The methodology for DNL determination needs further validation against standard methodologie...
Sources of hepatic glucose production by 2H2O ingestion and Bayesian analysis o...
Delgado, T. C.; Barosa, C.; Castro, M. M. C. A.; Geraldes, C. F. G. C.; Bastos, M.; Baptista, C.; Fagulha, A.; Barros, L.; Mota, A.; Carvalheiro, M.The contribution of gluconeogenesis to hepatic glucose production (GP) was quantified after 2H2O ingestion by Bayesian analysis of the position 2 and 5 2H-NMR signals (H2 and H5) of monoacetone glucose (MAG) derived from urinary acetaminophen glucuronide. Six controls and 10 kidney transplant (KTx) patients with cyclosporine A (CsA) immunosuppressant therapy were studied. Seven KTx patients were lean and euglyc...
First in vivo MRI assessment of a self-assembled metallostar compound endowed w...
Livramento, J. B.; Weidensteiner, C.; Prata, M. I. M.; Allegrini, P. R.; Geraldes, C. F. G. C.; Helm, L.; Kneuer, R.; Merbach, A. E.; Santos, A. C.Fe[Gd2bpy(DTTA)2(H2O)4]34- is a self-assembled, metallostar-structured potential MRI contrast agent, with six efficiently relaxing Gd3+ centres confined into a small molecular space. Its proton relaxivity is particularly remarkable at very high magnetic fields (r1 = 15.8 mM-1 s-1 at 200 MHz, 37°C, in H2O). Here we report the first in vivo MRI feasibility study, complemented with dynamic gamma scintigraphic imag...
Targeting of lanthanide(III) chelates of DOTA-type glycoconjugates to the hepat...
Prata, M. I. M.; Santos, A. C.; Torres, S.; André, J. P.; Martins, J. A.; Neves, M.; García-Martín, M. L.; Rodrigues, T. B.; López-Larrubia, P.The characterization of a new class of hydrophilic liver-targeted agents for gamma-scintigraphy and MRI, consisting, respectively, of [153Sm]3+ or Gd3+ complexes of DOTA monoamide or bisamide linked glycoconjugates (DOTA = 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid), is reported. In vitro studies show high uptake of radiolabeled [153Sm]-DOTAGal2 by the human hepatocyte carcinoma cell line Hep G2 c...
Effects of Li+ transport and intracellular binding on Li+/Mg2+ competition in b...
Fonseca, C. P.; Montezinho, L. P.; Nabais, C.; Tomé, A. R.; Freitas, H.; Geraldes, C. F. G. C.; Castro, M. M. C. A.Li+ transport, intracellular immobilisation and Li+/Mg2+ competition were studied in Li+-loaded bovine chromaffin cells. Li+ influx rate constants, ki, obtained by atomic absorption (AA) spectrophotometry, in control (without and with ouabain) and depolarising (without and with nitrendipine) conditions, showed that L-type voltage-sensitive Ca2+ channels have an important role in Li+ uptake under depolarising co...
Intracellular lithium and cyclic AMP levels are mutually regulated in neuronal ...
Montezinho, L. P.; Duarte, C. B.; Fonseca, C. P.; Glinka, Y.; Layden, B.; Freitas, D. Mota de; Geraldes, C. F. G. C.; Castro, M. M. C. A.In this work, we studied the effect of intracellular 3',5'-cyclic adenosine monophosphate (cAMP) on Li+ transport in SH-SY5Y cells. The cells were stimulated with forskolin, an adenylate cyclase activator, or with the cAMP analogue, dibutyryl-cAMP. It was observed that under forskolin stimulation both the Li+ influx rate constant and the Li+ accumulation in these cells were increased. Dibutyryl-cAMP also increa...
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