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Anterior positioning of sex chromosomes on the head of human sperm sorted using...
Alçada-Morais, Sofia; Sousa, Ana Paula; Paiva, Artur; Almeida-Santos, Teresa; Ramalho-Santos, J.The human ejaculate contains subpopulations of sperm with distinct properties. Human X- and Y-bearing sperm were separated with fluorescence activated cell sorting. To avoid the use of UV light the quantitative DNA dyes DRAQ5® and Dyecycle™ Vybrant® Violet were used. Sorting efficiency was similar for both dyes, but lower than what is usually obtained with the classical method involving Hoechst 33342 and UV lig...
Data: 2013
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Origem: Estudo Geral - Universidade de Coimbra
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