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Chemical composition and antioxidant activity of sulphated polysaccharides extr...
Rodríguez-Jasso, Rosa María; Mussatto, Solange I.; Pastrana, Lorenzo; Aguilar, Cristóbal N.; Teixeira, J. A.Sulphated polysaccharides (SP) were extracted from Fucus vesiculosus seaweed by using two different hydrothermal processes: microwave-assisted extraction (MAE) and autohydrolysis (AH). The extraction yields, chemical composition, and antioxidant activity of the polysaccharides extracted were determined and compared. Although both processes afforded SP with similar yields (18.2 mass % and 16.5 mass %, for MAE an...
Extraction of sulfated polysaccharides by autohydrolysis of brown seaweed Fucus...
Rodríguez-Jasso, Rosa María; Mussatto, Solange I.; Pastrana, Lorenzo; Aguilar, Cristóbal N.; Teixeira, J. A.The extraction of sulfated polysaccharides (fucoidan) by autohydrolysis (AH) of brown seaweed Fucus vesiculosus was studied. Experimental assays were performed under different conditions of temperature (160 to 200°C) and reaction time (10 to 30 min) according to a 22 central composite design, and the conditions able to maximize the fucoidan yield were selected. The alga degradation and the total sugar yield in ...
Maximization of fructooligosaccharides and β-fructofuranosidase production by A...
Mussatto, Solange I.; Ballesteros, Lina F.; Martins, Silvia; Maltos, Dulce A .F.; Aguilar, Cristóbal N.; Teixeira, J. A.The conditions of temperature, moisture content, and inoculum rate able to maximize the production of fructooligosaccharides (FOS) and β-fructofuranosidase (FFase) enzyme by solid-state fermentation were established. Fermentation assays were performed using the support material (coffee silverskin) moistened to 60, 70, or 80 % with a 240-g/l sucrose solution and inoculated with a spore suspension of Aspergillus ...
Antibacterial activity of crude methanolic extract and fractions obtained from ...
Martins, Silvia; Amorim, Elba L. C.; Sobrinho, Tadeu J. S. Peixoto; Saraiva, Antonio M.; Pisciottano, Maria N. C.; Aguilar, Cristóbal N.The emergence of new infections and increase of bacteria drug-resistance rise up the urgent need for the development of new antibacterial agents from natural sources. This study was designed to evaluate the antibacterial activity of the crude methanolic extract (CME) and fractions [hexane (H), dichloromethane (DCM), ethyl acetate (EA) and ethanol (Et)] obtained from Larrea tridentata (Sessé & Moc. Ex DC.) Covil...
Bioactive compounds (phytoestrogens) recovery from Larrea tridentata leaves by ...
Martins, Silvia; Aguilar, Cristóbal N.; Teixeira, J. A.; Mussatto, Solange I.Methanol, ethanol, and acetone at four different concentrations (90%, 70%, 50%, and 30% v/v) were used for extraction of phytoestrogens (nordihydroguaiaretic acid (NDGA), kaempferol, and quercetin) from Larrea tridentata leaves. Besides the phytoestrogens extraction, the antioxidant potential, and the contents of total phenols, flavonoids, and protein in the produced extracts were also determined. The solvent a...
Antibacterial activity of crude methanolic extract and fractions obtained from ...
Martins, Silvia; Mussatto, Solange I.; Aguilar, Cristóbal N.; Amorim, Elba L. C.; Peixoto, Tadeu; Saraiva, Antonio M.; Teixeira, J. A.Larrea tridentata (Zygophyllaceae), commonly known as creosote bush, is a plant that grows in semiarid areas of Southwestern United States and Northern Mexico and was traditionally used for medicinal purposes. This plantis a notable source of natural compounds with approximately 50% of the leaves (dry weight) being extractable matter. This study was designed to evaluate the antibacterial activity of the crude m...
Physicochemical characterization and extraction of bioactive compound from Larr...
Martins, Silvia; Aguilar, Cristóbal N.; Mussatto, Solange I.; Teixeira, J. A.Este resumo faz parte de: Book of abstracts of the Meeting of the Institute for Biotechnology and Bioengineering, 2, Braga, Portugal, 2010. A versão completa do livro de atas está disponível em: http://hdl.handle.net/1822/10968
Production of Aspergillus niger GH1 tannase using solid-state fermentation
Cruz-Hernandez, M. A.; Contreras, J. C.; Lima, Nelson; Teixeira, J. A.; Aguilar, Cristóbal N.The production of tannase by Aspergillus niger GH1 in solid-state fermentation (SSF) was evaluated. Several concentrations of tannic acid (12.5, 25, 50 and 100 g/L) were tested in a model culture system. Column reactors were packed with polyurethane foam impregnated with liquid medium and inoculated with fungal spores. Tannase production was kinetically monitored by 48 h. Tannic acid uptake was spectrophotometr...
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