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Mixotrophic cultivation of Chlorella vulgaris using industrial dairy waste as o...
Abreu, Ana Paula; Fernandes, Bruno Daniel; Vicente, A. A.; Teixeira, J. A.; Dragone, GiulianoGrowth parameters and biochemical composition of the green microalga Chlorella vulgaris cultivated under different mixotrophic conditions were determined and compared to those obtained from a photoautotrophic control culture. Mixotrophic microalgae showed higher specific growth rate, final biomass concentration and productivities of lipids, starch and proteins than microalgae cultivated under photoautotrophic c...
Starch determination in Chlorella vulgaris-a comparison between acid and enzyma...
Fernandes, Bruno Daniel; Dragone, Giuliano; Abreu, Ana Paula; Geada, Pedro; Teixeira, J. A.; Vicente, A. A.Different methods for estimating starch in Chlorella vulgaris were compared with the view of establishing a procedure suitable for rapid and accurate determination of starch content in this microalgal species. A close agreement was observed between methods that use perchloric acid and enzymatic methods that use α-amylase and amyloglucosidase to hydrolyze the starch of microalgae grown under different nitrogen c...
Nutrient limitation as a strategy for increasing starch accumulation in microalgae
Dragone, Giuliano; Fernandes, Bruno Daniel; Abreu, Ana Paula; Vicente, A. A.; Teixeira, J. A.Increasing microalgal starch content by nutrient limitation has been regarded as an affordable approach for the production of third generation bioethanol. This work evaluated starch accumulation in Chlorella vulgaris P12 under different initial concentrations of nitrogen (0–2.2 g urea L−1) and iron (0–0.08 g FeNa-EDTA L−1) sources, using a central composite design (CCD) for two factors. The obtained model: Star...
Effect of nitrogen limitation on starch accumulation in Chlorella vulgaris
Dragone, Giuliano; Fernandes, Bruno Daniel; Abreu, Ana Paula; Vicente, A. A.; Teixeira, J. A.Due to the limited stocks of fossil fuels and the production of greenhouse gas carbon dioxide on their combustion alternative sources of energy are being investigated. Recently, microalgae have received much attention as a renewable energy resource because these photosynthetic microorganisms can convert sunlight, water and CO2 into potential biofuels (1). The microalga Chlorella vulgaris, particularly, has been...
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