Continuous hydrogen production rate from arabinose was significantly higher than from glucose, when higher organic loading rate was used. This fact was associated to higher lactate production in the reactor fed with glucose. The higher concentration of lactate was not a consequence of bacterial community shift, and is likely related to changes in the main metabolic pathways of glucose catabolism.
T. maritima and C. saccharolyticus co-culture (1:3) is advantageous for hydrogen production from mixture of glucose and xylose. The higher hydrogen production observed in co-culture was associated to lower lactic acid formation.
Background: Second generation hydrogen fermentation technologies using organic agricultural and forestry wastes are emerging. The efficient microbial fermentation of hexoses and pentoses resulting from the pretreatment of lingocellulosic materials is essential for the success of these processes. Results: Conversion of arabinose and glucose to hydrogen, by extreme thermophilic anaerobic mixed cultures was studie...
The development of new technologies for renewable energy production is crucial for decreasing the reliance in fossil fuels and improving global sustainability. Waste materials are valuable resources that can be used for the production of energy carriers. Organic wastes can be anaerobically digested to ultimately produce methane. Hydrogen can be recovered from this process, if methanogenesis is inhibited. These ...
Tese de doutoramento em Engenharia Química e Biológica ; Hydrogen is recognized as a potential future energy carrier for the replacement of fossil fuels. Mixed culture dark fermentation is considered one of the most promising, sustainable and environmentally friendly process for byohydrogen production, since waste and wastewaters can be used as feedstocks. This thesis reports batch and continuous experiments p...
Treatment of anaerobic granules with heat and two chemical treatments, contacting with 2-bromoethanesulfonate (BES) and with BESþChloroform, were applied to suppress hydrogen-consuming microorganisms. Three mesophilic expanded granular sludge bed (EGSB) reactors— RHeat, RBES, and RBESþChlo—were inoculated with the treated sludges and fed with synthetic sugar-based wastewater (5 gCOD L 1, HRT 20–12 h). Morpholog...
In the present study, two granular systems were compared in terms of hydrogen production rate, stability and bacterial diversity under extreme thermophilic conditions (70 °C). Two EGSB reactors were individually inoculated with heat treated methanogenic granules (HTG) and HTG amended with enrichment culture with high capacity of hydrogen production (engineered heat treated methanogenic granules – EHTG), respect...
Biohydrogen production from arabinose was examined using four different anaerobic sludges with different pHs ranging from 4.5 to 8.0. Arabinose (30 g l−1) was used as the substrate for all experiments. Individual cumulative hydrogen production data was used to estimate the three parameters of the modified Gompertz equation. Higher hydrogen production potentials were observed for higher pH values for all the slu...
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