Influência do cádmio em parâmetros bioquímicos e de crescimento de nabiças (Bra...

• Author(s): Barbosa, Patrícia Souza
• Date: 2011
• Persistent ID: http://hdl.handle.net/10400.5/4113
• Origin: Repositório da UTL
• Subject(s): turnip; Brassica rapa; cadmium; oxidative stress
• Document Type: Master Thesis
• Language: Portuguese
• Advisor(s): Martins, Maria Luísa Louro; Mourato, Miguel Barbosa

Description

Mestrado em Engenharia Alimentar - Instituto Superior de Agronomia In this work the response of Brassica rapa to cadmium induced oxidative stress was studied, both in hydroponic solution (with Cd concentrations of 10 and 25 μM) and in turf (10 μM). The following parameters were studied: plant length, number of leaves, fresh and dry weight of leaves and roots, mineral elements concentration (Cd, Na, K, Ca, Mg, Zn, Mn and Fe), MDA and H2O2 levels and chlorophyll content. The Cd contents in leaves were significantly higher in plants growing in hydroponic solution than in turfs, because Cd is more available in nutrient solution. The minerals mainly affected by Cd were Zn, Fe and Na, whose content in leaves decreased significantly when plants were grown in 25 μM Cd. In plants growing in 10 μM Cd, in hydroponic solution, an increase in H2O2 levels was detected. A higher level of lipid peroxidation was also detected in plants growing in Cd containing solution. It can be concluded that B. rapa plants, even when exposed to a concentration of 25 μM Cd, are not visually affected by Cd toxicity, although its leaves contain significant amounts of Cd, which can represent a danger for the consumer as the plants continue to look healthy.