Autor(es):
Brazão, João Miguel Henriques da Silva 
Data: 2010
Identificador Persistente: http://hdl.handle.net/10400.5/2973
Origem: Repositório da UTL
Assunto(s): rye; rDNA; chromatin; stress
Detalhes do Documento
Modulation of rye rDNA chromatin conformation induced by high temperature stress
Descrição
Report - Bolsa de integração na Investigação Epigenetic modulation has been correlated with expression patterns alterations involved in plant response to stress. However, the influence of abiotic stress, namely high temperature, in chromatin organization of plant nuclei is poorly known. The aim of this work was the analysis of rDNA chromatin organization in interphase nuclei of rye root-tip and coleoptiles from high temperature treated (HTS) and untreated plantlets, through Fluorescent In Situ Hybridization (FISH) using rDNA probes. Rye seeds were germinated in controlled conditions and HTS treated plantlets were subject to a period of 4 hours at 40oC. FISH was performed successfully using a new rDNA probe produced by PCR that can replace with advantage the pTa71 probe and the results obtained with both probes revealed significant differences in rDNA chromatin organization patterns between control and HTS in interphase nuclei from both tissues analyzed. The detected rDNA chromatin decondensation provoked by HTS may be involved in a greater potential for transcription necessary to the amendment induced by HTS and emphasize the role of chromatin epigenetic remodelling in plant adjustment pathways under stress conditions.
Report - Bolsa de integração na Investigação Epigenetic modulation has been correlated with expression patterns alterations involved in plant response to stress. However, the influence of abiotic stress, namely high temperature, in chromatin organization of plant nuclei is poorly known. The aim of this work was the analysis of rDNA chromatin organization in interphase nuclei of rye root-tip and coleoptiles from high temperature treated (HTS) and untreated plantlets, through Fluorescent In Situ Hybridization (FISH) using rDNA probes. Rye seeds were germinated in controlled conditions and HTS treated plantlets were subject to a period of 4 hours at 40oC. FISH was performed successfully using a new rDNA probe produced by PCR that can replace with advantage the pTa71 probe and the results obtained with both probes revealed significant differences in rDNA chromatin organization patterns between control and HTS in interphase nuclei from both tissues analyzed. The detected rDNA chromatin decondensation provoked by HTS may be involved in a greater potential for transcription necessary to the amendment induced by HTS and emphasize the role of chromatin epigenetic remodelling in plant adjustment pathways under stress conditions.
Tipo de Documento
Relatório
Idioma Inglês
Idioma Inglês
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