Microplate-based high throughput screening procedure for the isolation of lipid...

• Author(s): Pereira, Hugo ; Barreira, Luísa Paula Viola Afonso ; Mozes, Andre ; Florindo, C. ; Polo, Cristina ; Duarte, Catarina V ; Custódio, Luísa Margarida Batista ; Varela, J.
• Date: 2011
• Persistent ID: http://hdl.handle.net/10400.1/917
• Origin: Sapientia - Universidade do Algarve
• Document Type: Article
• Language: English

Description

Abstract We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.