Detalhes do Documento

Hepatic UDP-glucose 13C isotopomers from [U-13C]glucose: a simple analysis by 1...

Autor(es): Antunes, MJ cv logo 1 ; Biernat, M cv logo 2 ; Sola, E cv logo 3 ; Oliveira, L cv logo 4 ; Prieto, D cv logo 5 ; Franco, F cv logo 6 ; Providência, LA cv logo 7

Data: 2006

Identificador Persistente: http://hdl.handle.net/10400.4/506

Origem: Repositório do Centro Hospitalar e Universitário de Coimbra

Assunto(s): Espectroscopia de Ressonância Magnética; Fígado; Uridina Difosfato Glucose


Descrição
Menthol glucuronide was isolated from the urine of a healthy 70-kg female subject following ingestion of 400 mg of peppermint oil and 6 g of 99% [U-(13)C]glucose. Glucuronide (13)C-excess enrichment levels were 4-6% and thus provided high signal-to-noise ratios (SNRs) for confident assignment of (13)C-(13)C spin-coupled multiplet components within each (13)C resonance by (13)C NMR. The [U-(13)C]glucuronide isotopomer derived via direct pathway conversion of [U-(13)C]glucose to [U-(13)C]UDP-glucose was resolved from [1,2,3-(13)C(3)]- and [1,2-(13)C(2)]glucuronide isotopomers derived via Cori cycle or indirect pathway metabolism of [U-(13)C]glucose. In a second study, a group of four overnight-fasted patients (63 +/- 10 kg) with severe heart failure were given peppermint oil and infused with [U-(13)C]glucose for 4 hr (14 mg/kg prime, 0.12 mg/kg/min constant infusion) resulting in a steady-state plasma [U-(13)C]glucose enrichment of 4.6% +/- 0.6%. Menthol glucuronide was harvested and glucuronide (13)C-isotopomers were analyzed by (13)C NMR. [U-(13)C]glucuronide enrichment was 0.6% +/- 0.1%, and the sum of [1,2,3-(13)C(3)] and [1,2-(13)C(2)]glucuronide enrichments was 0.9% +/- 0.2%. From these data, flux of plasma glucose to hepatic UDPG was estimated to be 15% +/- 4% that of endogenous glucose production (EGP), and the Cori cycle accounted for at least 32% +/- 10% of GP.
Tipo de Documento Artigo
Idioma Inglês
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