Lactose fermentation by recombinant Saccharomyces cerevisiae strains

• Author(s): Guimarães, Pedro M. R. ; Teixeira, J. A. ; Domingues, Lucília
• Date: 2008
• Persistent ID: http://hdl.handle.net/1822/9262
• Origin: RepositóriUM - Universidade do Minho
• Subject(s): Lactose fermentation; Cheese whey; Bio-ethanol; Recombinant S. cerevisiae
• Document Type: Conference Object
• Language: English

Description

The development of Saccharomyces cerevisiae strains with the ability to ferment lactose has a high biotechnological interest, particularly for cheese whey bioremediation processes with simultaneous bio-ethanol production. We have developed a flocculent S. cerevisiae strain that efficiently ferments lactose to ethanol, using a combination of genetic engineering and evolutionary engineering approaches. This strain fermented efficiently and nearly completely (residual lactose < 3 g·L -1) lactose concentrations up to 150 g·L-1, including 3-fold concentrated cheese whey, producing ethanol titres up to 8% (v/v). The ethanol productivity obtained with this strain (> 1.5 g·L -1·h-1) was higher than that reported for batch or fed-batch fermentations with other lactose-consuming recombinant S. cerevisiae strains. The strain is highly flocculent, a property that makes it interesting for the development of high cell density fermentation processes, which may attain much higher productivity.