Hyperosmotic stress induces metacaspase - and mitochondria - dependent apoptosi...

• Author(s): Silva, Rui D. ; Sotoca, Roberto ; Johansson, Björn ; Ludovico, Paula ; Sansonetty, Filipe ; Silva, Manuel T. ; Peinado, José M. ; Côrte-Real, Manuela
• Date: 2005
• Persistent ID: http://hdl.handle.net/1822/4353
• Origin: RepositóriUM - Universidade do Minho
• Subject(s): Programmed cell death; Cytochrome c; Yeast; Ultrastructural changes during apoptosis
• Document Type: Article
• Language: English

Description

Prova tipográfica (In Press) During the last years, several reports described an apoptosis-like programmed cell death process in yeast in response to different environmental aggressions. Here, evidence is presented that hyperosmotic stress induces in Saccharomyces cerevisiae a cell death process accompanied by morphological and biochemical indicators of apoptotic programmed cell death, namely chromatin condensation along the nuclear envelope, mitochondrial swelling and reduction of cristae number, production of reactive oxygen species and DNA strand breaks, with maintenance of plasma membrane integrity. Disruption of AIF1 had no effect on cell survival, but lack of Yca1p drastically reduced metacaspase activation and decreased cell death indicating that this death process was associated to activation of this protease. Supporting the involvement of mitochondria and cytochrome c in caspase activation, the mutant strains cyc1Δ cyc7Δ and cyc3Δ, both lacking mature cytochrome c, displayed a decrease in caspase activation associated to increased cell survival when exposed to hyperosmotic stress. These findings indicate that hyperosmotic stress triggers S. cerevisiae into an apoptosis-like programmed cell death that is mediated by a caspase-dependent mitochondrial pathway partially dependent on cytochrome c.