Image analysis, methanogenic activity and molecular biological techniques to mo...

• Author(s): Pereira, M. A. ; Roest, K. ; Stams, A. J. M. ; Akkermans, A. D. L. ; Amaral, A. L. ; Pons, M. N. ; Ferreira, E. C. ; Mota, M. ; Alves, M. M.
• Date: 2003
• Persistent ID: http://hdl.handle.net/1822/2595
• Origin: RepositóriUM - Universidade do Minho
• Subject(s): Long Chain Fatty Acids; EGSB reactor; Granular sludge; Image analysis; Molecular techniques; Methanogenic activity
• Document Type: Conference Object
• Language: English

Description

Morphological changes in anaerobic granular sludge fed with increasing loads of oleic acid were quantified by image analysis. The combination of this technique with data on the accumulation of adsorbed long chain fatty acid give insight into the mechanisms of sludge disintegration, flotation and washout The molecular characterization of microbial community indicated that the bacterial domain was affected during sludge disintegration, flotation and washout. The archaeal domain was less affected although no acetoclastic and only a residual hydrogenotrophic activity were detected at the end of the operation.